Interaction between citrate synthase and thiolase

B. Sumegi, H. F. Gilbert, P. A. Srere

Research output: Contribution to journalArticle

26 Citations (Scopus)


Thiolase, a mitochondrial matrix enzyme which produces CoASAc from fatty acids, is shown to interact with citrate synthase, the mitochondrial matrix enzyme responsible for CoASAc utilization. The interaction is demonstrated in three ways: the two enzymes co-precipitate in polyethylene glycol; thiolase causes a change in the fluorescence anisotropy of labeled citrate synthase; and the two enzymes co-elute in gel permeation chromatography. The interactions are shown to be specific by the use of enzyme not metabolically related to citrate synthase.

Original languageEnglish
Pages (from-to)188-190
Number of pages3
JournalJournal of Biological Chemistry
Issue number1
Publication statusPublished - Jan 1 1985

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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