Interaction between citrate synthase and malate dehydrogenase: Substrate channeling of oxaloacetate

Igor Morgunov, P. Srere

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

The interactions between pig heart citrate synthase and mitochondrial malate dehydrogenase or cytosolic malate dehydrogenase were studied using the frontal analysis method of gel filtration and by precipitation in polyethylene glycol. This method showed that an interaction between citrate synthase and mitochondrial malate dehydrogenase occurred but no interaction between citrate synthase and cytosolic malate dehydrogenase. Channeling of oxaloacetate in the malate dehydrogenase and citrate synthase-coupled systems was tested using polyethylene glycol precipitates of citrate synthase and mitochondrial malate dehydrogenase, and citrate synthase and cytosolic malate dehydrogenase. The effectiveness of large amounts of aspartate aminotransferase and oxaloacetate decarboxylase, as competing enzymes for the intermediate oxaloacetate, was examined. Aspartate aminotransferase and oxaloacetate decarboxylase were less effective competitors for oxaloacetate when precipitated citrate synthase and mitochondrial malate dehydrogenase in polyethylene glycol was used at low ionic strength compared with free enzymes in the absence of polyethylene glycol or with a co-precipitate of citrate synthase and cytosolic malate dehydrogenase. Substrate channeling of oxaloacetate with citrate synthase-mitochondrial malate dehydrogenase precipitate was inefficient at high ionic strength. These effects could be explained through electrostatic interactions of mitochondrial but not cytosolic malate dehydrogenase with citrate synthase.

Original languageEnglish
Pages (from-to)29540-29544
Number of pages5
JournalJournal of Biological Chemistry
Volume273
Issue number45
DOIs
Publication statusPublished - Nov 6 1998

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Oxaloacetic Acid
Citrate (si)-Synthase
Malate Dehydrogenase
Substrates
aspartate 4-decarboxylase
oxaloacetate decarboxylase
Malate Synthase
Precipitates
Aspartate Aminotransferases
Ionic strength
Osmolar Concentration
Enzymes
Coulomb interactions
Static Electricity
Gel Chromatography
Swine
Gels

ASJC Scopus subject areas

  • Biochemistry

Cite this

Interaction between citrate synthase and malate dehydrogenase : Substrate channeling of oxaloacetate. / Morgunov, Igor; Srere, P.

In: Journal of Biological Chemistry, Vol. 273, No. 45, 06.11.1998, p. 29540-29544.

Research output: Contribution to journalArticle

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