Interaction between cholesterol and non-ionic surfactants studied by thin-layer chromatography

E. Forgács, T. Cserháti, O. Farkas, A. Eckhardt, I. Miksik, Z. Deyl

Research output: Contribution to journalArticle

5 Citations (Scopus)


The interaction between non-ionic surfactants and cholesterol has been studied by reversed-phase thin-layer chromatography (RP-TLC) using cholesterol impregnated TLC plates and methanol-water mixtures as mobile phases. The R M values obtained were in linear correlation with the methanol concentration of the mobile phase. The intercept obtained from linear regression analysis (RMo), being characteristic for the strength of interaction, and the slope (b), being related to the surface area of surfactants in contact with cholesterol, have been determined. Stepwise regression analysis (SRA) was performed to find relationship between the structural parameters of surfactants and strength of interaction. The results show that stacking interaction exists between cholesterol and the aromatic ring of the surfactants. The number of ethylene oxide units and length of the carbon chain in the surfactant molecules have significant effect on the strength of the interaction between the compounds studied.

Original languageEnglish
Pages (from-to)1981-1992
Number of pages12
JournalJournal of Liquid Chromatography and Related Technologies
Issue number13
Publication statusPublished - Aug 26 2004


  • Cholesterol
  • Interaction
  • Non-ionic surfactants
  • Thin-layer chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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