Interaction between ATP-sensitive K+ channels and nitric oxide on pial arterioles in piglets

F. Bari, Robert A. Errico, Thomas M. Louis, David W. Busija

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The interaction between ATP-sensitive K+ channels (K(ATP)) and nitric oxide (NO) was studied in pial arterioles of piglets. We examined the effects of N(ω)-nitro-L-arginine methyl ester (L-NAME), a general inhibitor of nitric oxide synthase (NOS), and 7-nitroindazole (7-NI), a selective inhibitor of neuronal NOS, on aprikalim-induced cerebral vasodilation. Topically applied, aprikalim, a selective activator of K(ATP), dilated arterioles by 11 ± 7% at 10-8 M and 17 ± 6% at 10-6 M. After L-NAME treatment (15 mg/kg, i.v.), the response was reduced (4 ± 4% and 12 ± 7%, respectively; n = 8, p <0.05). Administration of 7-NI (50 mg/kg, i.p.) did not change pial arteriolar responsiveness to aprikalim. However, both L-NAME and 7-NI reduced the vasodilator responses to 10-4 M N-methyl-D-aspartate (NMDA) (by 73% and by 36%, respectively). Furthermore, 7-NI treatment abolished the glutamate-induced dilatation of pial arterioles. Administration of L-NAME reduced the NOS activity in the cerebral cortex by 88%, whereas the reduction after the 7-NI treatment was 44%. Pretreatment and coadministration of 10-5 M glibenclaminde, a specific inhibitor of K(ATP) or L-NAME administration, did not change the dilatory response to sodium nitroprusside. We conclude that NO may be involved in aprikalim-induced dilation of pial arterioles.

Original languageEnglish
Pages (from-to)1158-1164
Number of pages7
JournalJournal of Cerebral Blood Flow and Metabolism
Volume16
Issue number6
Publication statusPublished - 1996

Fingerprint

NG-Nitroarginine Methyl Ester
Arterioles
Nitric Oxide
Adenosine Triphosphate
Nitric Oxide Synthase
Dilatation
Nitric Oxide Synthase Type I
Nitroprusside
N-Methylaspartate
Vasodilator Agents
Vasodilation
Cerebral Cortex
Glutamic Acid
7-nitroindazole
aprikalim

Keywords

  • Aprikalim
  • Glibenclamide
  • Glutamate
  • Nitric oxide synthase
  • NMDA

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Interaction between ATP-sensitive K+ channels and nitric oxide on pial arterioles in piglets. / Bari, F.; Errico, Robert A.; Louis, Thomas M.; Busija, David W.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 16, No. 6, 1996, p. 1158-1164.

Research output: Contribution to journalArticle

Bari, F. ; Errico, Robert A. ; Louis, Thomas M. ; Busija, David W. / Interaction between ATP-sensitive K+ channels and nitric oxide on pial arterioles in piglets. In: Journal of Cerebral Blood Flow and Metabolism. 1996 ; Vol. 16, No. 6. pp. 1158-1164.
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