Inter-laboratory comparison of Cry1Ab toxin quantification in MON 810 maize by enzyme-immunoassay

A. Székács, Gabriele Weiss, David Quist, Eszter Takács, Béla Darvas, Matthias Meier, Trilochan Swain, Angelika Hilbeck

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A laboratory ring trial was performed in four laboratories for determination of Cry1Ab toxin in leaf material of MON 810 maize using a standardised enzyme-linked immunoassay protocol. Statistical analysis was carried out by the ISO 5725-2 guidelines, sample standard deviation and standard error, within-laboratory and inter-laboratory SD and SE were calculated. Measured inter-laboratory average values were 12.5±4.0, 15.3±4.6 and 72.6±17.8 μg/g for three lyophilised samples, and 27.8±4.3 μg/g for a frozen sample, yet, Cry1Ab concentrations ranged 66.5-160.1% of the corresponding IA. Determined concentrations by in-house protocols were statistically not different in one laboratory and different in two laboratories from the corresponding values by the joint protocol. Results emphasise the importance of a standardised protocol among laboratories for comparable quantitative Cry1Ab toxin determination. However, even when using a standardised protocol, significant differences still occur among toxin concentrations detected in different laboratories, although with a smaller range of variation.

Original languageEnglish
Pages (from-to)99-121
Number of pages23
JournalFood and Agricultural Immunology
Volume23
Issue number2
DOIs
Publication statusPublished - Jun 2012

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enzyme immunoassays
Immunoenzyme Techniques
Zea mays
toxins
corn
immunoassays
sampling
statistical analysis
Guidelines
enzymes

Keywords

  • Bacillus thuringiensis
  • Cry1Ab toxin
  • ELISA
  • GM plants
  • MON 810

ASJC Scopus subject areas

  • Food Science
  • Immunology
  • Agronomy and Crop Science

Cite this

Inter-laboratory comparison of Cry1Ab toxin quantification in MON 810 maize by enzyme-immunoassay. / Székács, A.; Weiss, Gabriele; Quist, David; Takács, Eszter; Darvas, Béla; Meier, Matthias; Swain, Trilochan; Hilbeck, Angelika.

In: Food and Agricultural Immunology, Vol. 23, No. 2, 06.2012, p. 99-121.

Research output: Contribution to journalArticle

Székács, A. ; Weiss, Gabriele ; Quist, David ; Takács, Eszter ; Darvas, Béla ; Meier, Matthias ; Swain, Trilochan ; Hilbeck, Angelika. / Inter-laboratory comparison of Cry1Ab toxin quantification in MON 810 maize by enzyme-immunoassay. In: Food and Agricultural Immunology. 2012 ; Vol. 23, No. 2. pp. 99-121.
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