Intensity interrogation near cutoff resonance for label-free cellular profiling

Yousef Nazirizadeh, Volker Behrends, Aurél Prósz, Norbert Orgovan, R. Horváth, Ann M. Ferrie, Ye Fang, Christine Selhuber-Unkel, Martina Gerken

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We report a method enabling intensity-based readout for label-free cellular assays, and realize a reader device with the same footprint as a microtiter plate. For unambiguous resonance intensity measurements in resonance waveguide grating (RWG) sensors, we propose to apply resonances near the substrate cutoff wavelength. This method was validated in bulk refractive index, surface bilayer and G protein-coupled receptor (GPCR) experiments. The significantly reduced size of the reader device opens new opportunities for easy integration into incubators or liquid handling systems.

Original languageEnglish
Article number24685
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - Apr 18 2016

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Incubators
Equipment and Supplies
Refractometry
G-Protein-Coupled Receptors
Membrane Proteins

ASJC Scopus subject areas

  • General

Cite this

Nazirizadeh, Y., Behrends, V., Prósz, A., Orgovan, N., Horváth, R., Ferrie, A. M., ... Gerken, M. (2016). Intensity interrogation near cutoff resonance for label-free cellular profiling. Scientific Reports, 6, [24685]. https://doi.org/10.1038/srep24685

Intensity interrogation near cutoff resonance for label-free cellular profiling. / Nazirizadeh, Yousef; Behrends, Volker; Prósz, Aurél; Orgovan, Norbert; Horváth, R.; Ferrie, Ann M.; Fang, Ye; Selhuber-Unkel, Christine; Gerken, Martina.

In: Scientific Reports, Vol. 6, 24685, 18.04.2016.

Research output: Contribution to journalArticle

Nazirizadeh, Y, Behrends, V, Prósz, A, Orgovan, N, Horváth, R, Ferrie, AM, Fang, Y, Selhuber-Unkel, C & Gerken, M 2016, 'Intensity interrogation near cutoff resonance for label-free cellular profiling', Scientific Reports, vol. 6, 24685. https://doi.org/10.1038/srep24685
Nazirizadeh, Yousef ; Behrends, Volker ; Prósz, Aurél ; Orgovan, Norbert ; Horváth, R. ; Ferrie, Ann M. ; Fang, Ye ; Selhuber-Unkel, Christine ; Gerken, Martina. / Intensity interrogation near cutoff resonance for label-free cellular profiling. In: Scientific Reports. 2016 ; Vol. 6.
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