Integrative promoter cloning plasmid vectors for Rhizobium meliloti

P. Élo, Sz Semsey, A. Kereszt, T. Nagy, P. Papp, L. Orosz

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A set of integrative 'promoter probe' plasmids were constructed for both translational and transcriptional fusions. The vectors are based oil the broad host range, low copy number plasmid pRK290 (IncP1) in which the attachment site of Rhizobium phage 16-3 and the lacZ gene of Escherichia coli were combined. The vectors integrate into the chromosome of Rhizobium meliloti, providing also the advantages of the single copy promoter probe cassettes. Thus they fulfil the prerequisite of the systems used for investigating gene regulation. The plasmids were applied for the study of the transcription regulation of the 16-3 phage. Their versatile use is also demonstrated.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalFEMS microbiology letters
Volume159
Issue number1
DOIs
Publication statusPublished - Feb 1 1998

Keywords

  • Attachment site
  • Gene fusion
  • Promoter cloning vector
  • Rhizobium meliloti
  • Site-specific recombination

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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