Insulin uptake, localization and production in previously insulin treated and untreated Tetrahymena. Data on the mechanism of hormonal imprinting

G. Csaba, P. Kovács

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Confocal microscopic experiments demonstrate the presence of insulin in Tetrahymena, observed also in earlier experiments. However, there is a broad spectrum of insulin-containing cells from the immunocytochemically insulin-free, to the strongly antibody-reactive ones. During 1 h of insulin treatment (imprinting) the cells gradually bind and take up insulin, and the process is slow. One minute after the start of treatment there is no difference in the number of insulin antibody-reactive cells and amount of insulin. After 5 or 10 min the cells bind and contain more insulin and after 1 h most of the cells are densely packed with the insulin antibody-reactive material. Insulin imprinting accelerates binding and uptake alike: 48 h after imprinting and 1 min after the start of the second treatment, more insulin is present on the surface and inside the cells, than after 10 min in the first-time treated cells. Theoretically, this effect of hormonal imprinting helps to maintain the species by facilitating molecular recognition and binding as well as uptake of useful molecules. The experiments also support previous observations on the parallel receptor-evoking (strengthening) and hormone-producing effect of hormonal imprinting. Copyright (C) 2000 John Wiley and Sons, Ltd.

Original languageEnglish
Pages (from-to)161-167
Number of pages7
JournalCell Biochemistry and Function
Volume18
Issue number3
DOIs
Publication statusPublished - Sep 2000

Fingerprint

Tetrahymena
Insulin
Insulin Antibodies
Cells
Molecular recognition
Experiments
Hormones
Molecules

Keywords

  • Hormonal imprinting
  • Insulin localization
  • Insulin production
  • Insulin storage
  • Receptor
  • Tetrahymena pyriformis

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

Cite this

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