Insulin treatment of Tetrahymena pyriformis resulted in a long-lasting increase in the insulin content of the cells. After about 200 generations, insulin levels significantly higher than in the control were demonstrated by using a quantitative immunocytochemical method. Although the insulin content fluctuated from day to day, it was always higher in the insulin pre-treated (imprinted) cells than in the controls. The results emphasise the simultaneous stimulation of hormone and receptor formation by hormonal imprinting.
ASJC Scopus subject areas
- Cell Biology