Insulin resistance in an animal model of polycystic ovary disease is aggravated by vitamin D deficiency: Vascular consequences

Leila Hadjadj, Szabolcs Várbíró, Eszter Mária Horváth, Anna Monori-Kiss, Éva Pál, Gellért Balázs Karvaly, Andrea Heinzlmann, Attila Magyar, István Szabó, Réka Eszter Sziva, Zoltán Benyó, Mariann Buday, György László Nádasy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Hyperandrogenic state in females is accompanied with metabolic syndrome, insulin resistance and vascular pathologies. A total of 67%–85% of hyperandrogenic women suffer also from vitamin D deficiency. We aimed to check a potential interplay between hyperandrogenism and vitamin D deficiency in producing insulin resistance and effects on coronary resistance arteries. Adolescent female rats were divided into four groups, 11–12 animals in each. Transdermal testosterone-treated and vehicle-treated animals were kept either on vitamin D-deficient or on vitamin D-supplemented diet for 8 weeks. Plasma sexual steroid, insulin, leptin and vitamin D plasma levels were measured, and oral glucose tolerance test was performed. In coronary arterioles, insulin receptor and vitamin D receptor expressions were tested by immunohistochemistry, and insulin-induced relaxation was measured in vitro on isolated coronary resistance artery segments. Testosterone impaired glucose tolerance, and it diminished insulin relaxation but did not affect the expression of insulin and vitamin D receptors in vascular tissue. Vitamin D deficiency elevated postprandial insulin levels and homeostatic model assessment insulin resistance. It also diminished insulin-induced coronary arteriole relaxation, while it raised the expression of vitamin D and insulin receptors in the endothelial and medial layers. Our conclusion is that both hyperandrogenism and vitamin D deficiency reduce sensitivity of coronary vascular tissue to insulin, but they do it with different mechanisms.

Original languageEnglish
JournalDiabetes and Vascular Disease Research
DOIs
Publication statusAccepted/In press - Feb 1 2018

Fingerprint

Vitamin D Deficiency
Blood Vessels
Insulin Resistance
Ovary
Animal Models
Insulin
Calcitriol Receptors
Vitamin D
Hyperandrogenism
Insulin Receptor
Arterioles
Testosterone
Coronary Vessels
Glucose Intolerance
Glucose Tolerance Test
Leptin
Immunohistochemistry
Steroids
Pathology
Diet

Keywords

  • androgen excess
  • bicomponent model
  • coronary arteriole
  • insulin resistance
  • Vitamin D deficiency

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Cardiology and Cardiovascular Medicine

Cite this

Insulin resistance in an animal model of polycystic ovary disease is aggravated by vitamin D deficiency : Vascular consequences. / Hadjadj, Leila; Várbíró, Szabolcs; Horváth, Eszter Mária; Monori-Kiss, Anna; Pál, Éva; Karvaly, Gellért Balázs; Heinzlmann, Andrea; Magyar, Attila; Szabó, István; Sziva, Réka Eszter; Benyó, Zoltán; Buday, Mariann; Nádasy, György László.

In: Diabetes and Vascular Disease Research, 01.02.2018.

Research output: Contribution to journalArticle

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AU - Monori-Kiss, Anna

AU - Pál, Éva

AU - Karvaly, Gellért Balázs

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AU - Magyar, Attila

AU - Szabó, István

AU - Sziva, Réka Eszter

AU - Benyó, Zoltán

AU - Buday, Mariann

AU - Nádasy, György László

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