Insulin induced translocation of Na+ /K+-ATPase is decreased in the heart of streptozotocin diabetic rats

Klara Rosta, Eszter Tulassay, Anna Enzsoly, Katalin Ronai, Ambrus Szantho, Tamas Pandics, Andrea Fekete, Peter Mandl, A. Vér

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Aim: To investigate the effect of acute insulin administration on the subcellular localization of Na+/K+-ATPase isoforms in cardiac muscle of healthy and streptozotocin-induced diabetic rats. Methods: Membrane fractions were isolated with subcellular fractionation and with cell surface biotinylation technique. Na+/K+-ATPase subunit isoforms were analysed with ouabain binding assay and Western blotting. Enzyme activity was measured using 3-O-methylfluorescein-phosphatase activity. Results: In control rat heart muscle α1 isoform of Na+/K+ ATPase resides mainly in the plasma membrane fraction, while α2 isoform in the intracellular membrane pool. Diabetes decreased the abundance of α1 isoform (25 %, P+/K +-ATPase isoforms in the heart. α2 isoform translocation is the most vulnerable to the reduced insulin response in diabetes. α1 isoform also translocates in response to insulin treatment in healthy rat. Insulin mediates Na+/K+-ATPase α1-and α2-subunit translocation to the cardiac muscle plasma membrane via a PI3-kinase-dependent mechanism.

Original languageEnglish
Pages (from-to)1616-1624
Number of pages9
JournalActa Pharmacologica Sinica
Volume30
Issue number12
DOIs
Publication statusPublished - Dec 2009

Fingerprint

Streptozocin
Protein Isoforms
Insulin
Myocardium
Cell Membrane
Biotinylation
Cell Fractionation
Intracellular Membranes
sodium-translocating ATPase
Ouabain
Phosphatidylinositol 3-Kinases
Adenosine Triphosphatases
Western Blotting
Membranes
Enzymes

Keywords

  • Na/K -AtPase isoenzyme
  • Rat cardiac muscle
  • Redistribution
  • Streptozotocin-diabetes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Cite this

Insulin induced translocation of Na+ /K+-ATPase is decreased in the heart of streptozotocin diabetic rats. / Rosta, Klara; Tulassay, Eszter; Enzsoly, Anna; Ronai, Katalin; Szantho, Ambrus; Pandics, Tamas; Fekete, Andrea; Mandl, Peter; Vér, A.

In: Acta Pharmacologica Sinica, Vol. 30, No. 12, 12.2009, p. 1616-1624.

Research output: Contribution to journalArticle

Rosta, K, Tulassay, E, Enzsoly, A, Ronai, K, Szantho, A, Pandics, T, Fekete, A, Mandl, P & Vér, A 2009, 'Insulin induced translocation of Na+ /K+-ATPase is decreased in the heart of streptozotocin diabetic rats', Acta Pharmacologica Sinica, vol. 30, no. 12, pp. 1616-1624. https://doi.org/10.1038/aps.2009.162
Rosta, Klara ; Tulassay, Eszter ; Enzsoly, Anna ; Ronai, Katalin ; Szantho, Ambrus ; Pandics, Tamas ; Fekete, Andrea ; Mandl, Peter ; Vér, A. / Insulin induced translocation of Na+ /K+-ATPase is decreased in the heart of streptozotocin diabetic rats. In: Acta Pharmacologica Sinica. 2009 ; Vol. 30, No. 12. pp. 1616-1624.
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