Insulin-degrading enzyme exists inside of rat liver peroxisomes and degrades oxidized proteins

Masashi Morita, Igor V. Kurochkin, Kiyoto Motojima, Sataro Goto, Tatsuya Takano, Shoji Okamura, Ryuichiro Sato, Sadaki Yokota, Tsuneo Imanaka

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53 Citations (Scopus)

Abstract

Insulin-degrading enzyme (IDE) was detected by immunoblot analysis in highly purified rat liver peroxisomes. IDE in the peroxisomal fraction was resistant to proteolysis by trypsin and chymotrypsin under conditions where the peroxisomal membranes remained intact. After sonication of the peroxisomal fraction, IDE was recovered in the supernatant fraction. Further, the localization of IDE in the peroxisomes was shown by immunoelectron microscopy. In addition, IDE isolated from peroxisomes degraded insulin as well as oxidized lysozyme as a model substrate for oxidized proteins. These results suggest that IDE exists in an active form in the matrix of rat liver peroxisomes and is involved in elimination of oxidized proteins in peroxisomes.

Original languageEnglish
Pages (from-to)309-315
Number of pages7
JournalCell Structure and Function
Volume25
Issue number5
DOIs
Publication statusPublished - Dec 1 2000

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Keywords

  • Insulin-degrading enzyme
  • Oxidized proteins
  • Peroxisomes
  • Proteolysis
  • Subcellular localization

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

Cite this

Morita, M., Kurochkin, I. V., Motojima, K., Goto, S., Takano, T., Okamura, S., Sato, R., Yokota, S., & Imanaka, T. (2000). Insulin-degrading enzyme exists inside of rat liver peroxisomes and degrades oxidized proteins. Cell Structure and Function, 25(5), 309-315. https://doi.org/10.1247/csf.25.309