Inosine-Arginine salt as a promising agent for in vitro activation of waterborne fish-pathogenic myxozoan actinospores

D. M. Kallert, B. Forró, E. Eszterbauer

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Mucus-derived nucleosides serve as key host cues for myxozoan actinospore fish host recognition, but to date their use for experimental actinospore activation in the laboratory or application in disease prevention has not progressed very far. One obstacle has been the low solubility of pure inosine and guanosine. To overcome this, we used inosine-Arginine salt (ino-Arg), which incorporates both high activation properties and high solubility. We tested its efficacy both in microassays directly observing reactions of actinospores of 2 distantly related myxozoan species, Myxobolus cerebralis and M. pseudodispar in comparison to inosine, as well as its actinospore-inactivation properties by premature polar capsule discharge in an infection experiment. Ino-Arg was considerably more effective in eliciting polar capsule discharge and sporoplasm emission at much lower concentrations than pure inosine and, in contrast to the latter, remained dissolved in aqueous solution. Ino-Arg exposure of M. pseudodispar actinospores resulted in polar capsule discharge and sporoplasm emission before host contact and subsequently in a lower infection rate in roach Rutilus rutilus.

Original languageEnglish
Pages (from-to)149-154
Number of pages6
JournalDiseases of Aquatic Organisms
Volume109
Issue number2
DOIs
Publication statusPublished - May 13 2014

    Fingerprint

Keywords

  • Actinospore activation
  • Aquaculture
  • Disease prevention
  • Myxozoa
  • Polar filament discharge
  • Whirling disease

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Cite this