Injury-enhanced calcium mobilization in circulating rat neutrophils models human PMN responses

Zoltan Fekete, Carl J. Hauser, John M. Adams, Charles A. Adams, Raquel M. Forsythe, G. Haskó, Da Zhong Xu, David H. Livingston, Edwin A. Deitch

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

G-protein coupled (GPC) chemoattractants are important neutrophil (PMN) activators in human shock and sepsis, acting in part by increasing cytosolic calcium ([Ca2+]i). Rats are widely used as laboratory models of shock and sepsis, but reports of [Ca2+]i flux in circulating rat PMN are rare. Moreover, the [Ca2+]i values reported often differ markedly from human systems. We developed study methods where basal [Ca2+]i values in circulating rat PMN were comparable to human PMN, but rat PMN still mobilized calcium poorly after stimulation. Trauma (laparotomy) did not change rat PMN basal [Ca2+]i, but induced brisk [Ca2+]i responses to chemokine and lipid mediators that approximated human PMN responses. This was associated with marked loading of microsomal calcium stores. Formyl peptides still mobilized calcium less well in rat than human PMN. Normal rat PMN appear to circulate in a less mature or primed form than human PMN. A very limited injury rapidly converts rat PMN to a more activated phenotype. PMN thus activated act quite similar to human PMN in terms of GPC receptor-mediated calcium mobilization. Trauma enhances rat PMN responses to GPC agonists at least in part by loading cell calcium stores.

Original languageEnglish
Pages (from-to)15-20
Number of pages6
JournalShock
Volume16
Issue number1
Publication statusPublished - Jul 2001

Fingerprint

Neutrophils
Calcium
Wounds and Injuries
GTP-Binding Proteins
Shock
Sepsis
Chemotactic Factors
G-Protein-Coupled Receptors
Chemokines
Laparotomy
Phenotype
Lipids
Peptides

Keywords

  • Cell calcium
  • Chemokines
  • fMLP
  • G proteins
  • GRO
  • MIP-2
  • Neutrophils
  • PAF
  • Rat
  • Thapsigargin

ASJC Scopus subject areas

  • Physiology
  • Critical Care and Intensive Care Medicine

Cite this

Fekete, Z., Hauser, C. J., Adams, J. M., Adams, C. A., Forsythe, R. M., Haskó, G., ... Deitch, E. A. (2001). Injury-enhanced calcium mobilization in circulating rat neutrophils models human PMN responses. Shock, 16(1), 15-20.

Injury-enhanced calcium mobilization in circulating rat neutrophils models human PMN responses. / Fekete, Zoltan; Hauser, Carl J.; Adams, John M.; Adams, Charles A.; Forsythe, Raquel M.; Haskó, G.; Xu, Da Zhong; Livingston, David H.; Deitch, Edwin A.

In: Shock, Vol. 16, No. 1, 07.2001, p. 15-20.

Research output: Contribution to journalArticle

Fekete, Z, Hauser, CJ, Adams, JM, Adams, CA, Forsythe, RM, Haskó, G, Xu, DZ, Livingston, DH & Deitch, EA 2001, 'Injury-enhanced calcium mobilization in circulating rat neutrophils models human PMN responses', Shock, vol. 16, no. 1, pp. 15-20.
Fekete Z, Hauser CJ, Adams JM, Adams CA, Forsythe RM, Haskó G et al. Injury-enhanced calcium mobilization in circulating rat neutrophils models human PMN responses. Shock. 2001 Jul;16(1):15-20.
Fekete, Zoltan ; Hauser, Carl J. ; Adams, John M. ; Adams, Charles A. ; Forsythe, Raquel M. ; Haskó, G. ; Xu, Da Zhong ; Livingston, David H. ; Deitch, Edwin A. / Injury-enhanced calcium mobilization in circulating rat neutrophils models human PMN responses. In: Shock. 2001 ; Vol. 16, No. 1. pp. 15-20.
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