Inhibitory effects of diclofenac on steroid glucuronidation in vivo do not affect hair-based doping tests for stanozolol

Gergely Zachár, Naved I.K. Deshmukh, Andrea Petróczi, Andrea D. Székely, Iltaf Shah, James Barker, Declan P. Naughton

Research output: Contribution to journalArticle

Abstract

In vitro studies show that diclofenac inhibits enzymatic steroid glucuronidation. This study was designed to investigate the influence of diclofenac on the excretion of stanozolol and 3-hydroxystanozolol via analyses in hair, blood and urine in vivo in a rat study. Brown Norway rats were administered with stanozolol (weeks 1–3) and diclofenac (weeks 1–6). Weekly assessment of steroid levels in hair was complemented with spot urine and serum tests. Levels of both stanozolol and 3-hydroxystanozolol steadily increased in hair during stanozolol treatment and decreased post-treatment, but remained readily detectable for 6 weeks. In contrast, compared to control rats, diclofenac significantly reduced urinary excretion of 3-hydroxystanozolol which was undetectable in most samples. This is the first report of diclofenac altering steroid metabolism in vivo, detrimentally affecting detection in urine, but not in hair, which holds considerable advantages over urinalysis for anti-doping tests.

Original languageEnglish
Article number976
JournalMolecules
Volume22
Issue number6
DOIs
Publication statusPublished - Jun 2017

Keywords

  • Anti-inflammatory drug
  • Inhibition
  • Metabolism
  • Steroid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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