Inhibitory effect of hypoxic condition on acetylcholine release is partly due to the effect of adenosine released from the tissue

E. Milusheva, B. Sperlágh, B. Kiss, L. Szporny, E. Pásztor, M. Papasova, E. Vízi

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Isolated longitudinal muscle strip with Auerbach's plexus attached was used to study the stimulation-evoked release of 3H-acetylcholine (3H-ACh) under normoxic and hypoxic conditions. Hypoxia reduced the release of ACh. Theophylline, a purinoceptor P1 antagonist and vinpocetine, an antiischemic compound partly reversed the effect of hypoxia. Unlike theophylline, the effect of vinpocetine was not mediated via adenosine action, since it failed to affect the presynaptic action of adenosine, and the effect of theophylline and vinpocetine was additive. When they were added together the effect of hypoxia was almost completely antagonized. Dipyridamole, an adenosine uptake inhibitor, potentiated the effect of hypoxia and the presynaptic inhibitory action of adenosine on ACh release. Evidence was obtained that the effect of hypoxia is at least partly due to adenosine formed from purine nucleotides.

Original languageEnglish
Pages (from-to)369-373
Number of pages5
JournalBrain Research Bulletin
Volume24
Issue number3
DOIs
Publication statusPublished - 1990

Fingerprint

vinpocetine
Adenosine
Acetylcholine
Theophylline
Purinergic P1 Receptor Antagonists
Purine Nucleotides
Myenteric Plexus
Dipyridamole
Hypoxia
Muscles

Keywords

  • Acetylcholine release
  • Adenosine
  • Hypoxia
  • Presynaptic modulation
  • Vinpocetine

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Inhibitory effect of hypoxic condition on acetylcholine release is partly due to the effect of adenosine released from the tissue. / Milusheva, E.; Sperlágh, B.; Kiss, B.; Szporny, L.; Pásztor, E.; Papasova, M.; Vízi, E.

In: Brain Research Bulletin, Vol. 24, No. 3, 1990, p. 369-373.

Research output: Contribution to journalArticle

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AU - Sperlágh, B.

AU - Kiss, B.

AU - Szporny, L.

AU - Pásztor, E.

AU - Papasova, M.

AU - Vízi, E.

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AB - Isolated longitudinal muscle strip with Auerbach's plexus attached was used to study the stimulation-evoked release of 3H-acetylcholine (3H-ACh) under normoxic and hypoxic conditions. Hypoxia reduced the release of ACh. Theophylline, a purinoceptor P1 antagonist and vinpocetine, an antiischemic compound partly reversed the effect of hypoxia. Unlike theophylline, the effect of vinpocetine was not mediated via adenosine action, since it failed to affect the presynaptic action of adenosine, and the effect of theophylline and vinpocetine was additive. When they were added together the effect of hypoxia was almost completely antagonized. Dipyridamole, an adenosine uptake inhibitor, potentiated the effect of hypoxia and the presynaptic inhibitory action of adenosine on ACh release. Evidence was obtained that the effect of hypoxia is at least partly due to adenosine formed from purine nucleotides.

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