Inhibitory effect of galanin on dopamine-induced enhanced vasopressin secretion in rat neurohypophyseal tissue cultures

M. Gálfi, L. Baláspiri, R. Tóth, I. Pávó, F. László, E. Morschl, C. Varga, F. László

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The effect of galanin (GAL) on vasopressin (VP) secretion was studied in 13-14-day cultures of isolated rat neurohypophyseal (NH) tissue. The VP content of the supernatant was determined by radioimmunoassay (RIA) after a 1- or 2-h incubation. A significantly decreased content of VP was detected following the administration of 10-6-10-9 M doses of GAL. Dopamine (DA) and the DA-active drugs apomorphine (APM) and Pro-Lys-Gly (PLG) (10-6 M in each medium) increased the VP level of NH tissue cultures. This VP concentration elevation could be blocked by the administration of GAL together with DA, APM or PLG. The DA-blocking effect of GAL was prevented by previous treatment with the GAL receptor antagonist galantid (M15). The results indicate that VP release is directly influenced by the GAL-ergic system. The GAL-ergic control of VP secretion from NH tissue in rats can occur independently of the hypothalamus, at the level of the posterior pituitary.

Original languageEnglish
Pages (from-to)17-23
Number of pages7
JournalRegulatory Peptides
Volume110
Issue number1
DOIs
Publication statusPublished - Dec 31 2002

Fingerprint

Galanin
Tissue culture
Vasopressins
Rats
Dopamine
Dopamine Agents
Apomorphine
Galanin Receptors
Tissue
Hypothalamus
Radioimmunoassay
Pharmaceutical Preparations

Keywords

  • Galanin regulation
  • Galanin-dopamine interaction
  • Galantid
  • Neurohypophyseal tissue culture
  • Vasopressin

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Inhibitory effect of galanin on dopamine-induced enhanced vasopressin secretion in rat neurohypophyseal tissue cultures. / Gálfi, M.; Baláspiri, L.; Tóth, R.; Pávó, I.; László, F.; Morschl, E.; Varga, C.; László, F.

In: Regulatory Peptides, Vol. 110, No. 1, 31.12.2002, p. 17-23.

Research output: Contribution to journalArticle

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