Inhibitory effect of galanin on adrenaline- and noradrenaline-induced increased oxytocin secretion in rat neurohypophyseal cell cultures

Marianna Radács, A. Molnár, F. László, C. Varga, Ferenc Láseló, M. Gálfi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The effects of the interactions between the 29 amino acid-containing peptide galanin and adrenaline or noradrenaline on the secretion of oxytocin were studied in 13- to 14-day cultures of isolated rat neurohypophyseal tissue. The α-receptor antagonist corynanthine blocked the adrenaline-induced increase of oxytocin secretion. When the β-receptor antagonist propranolol was added before the noradrenaline treatment, the antagonist prevented the noradrenaline-induced enhancement of oxytocin release. Following the addition of galanin, the extent of oxytocin secretion into the supernatant medium decreased. Adrenaline and noradrenaline treatments increased the oxytocin level. Preincubation with galanin reduced the adrenaline- and noradrenaline-induced oxytocin level elevations. The blocking effect of galanin was prevented by previous treatment with the galanin receptor antagonist galantid (M15). When adrenaline or noradrenaline treatment was applied before galanin addition, the oxytocin secretion remained enhanced. The present results indicate that the changes in oxytocin secretion induced by the adrenergic system can be directly influenced by the galaninergic system. The interactions between the adrenergic and galaninergic systems from the aspect of oxytocin secretion can occur at the level of the posterior pituitary, independently of the hypothalamus.

Original languageEnglish
Pages (from-to)59-66
Number of pages8
JournalJournal of Molecular Neuroscience
Volume42
Issue number1
DOIs
Publication statusPublished - Sep 2010

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Galanin
Oxytocin
Epinephrine
Norepinephrine
Cell Culture Techniques
Adrenergic Agents
Galanin Receptors
Yohimbine
Propranolol
Hypothalamus
Amino Acids
Peptides

Keywords

  • Adrenaline
  • Galanin
  • Neurohypophyseal cell culture
  • Noradrenaline
  • Oxytocin secretion

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

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title = "Inhibitory effect of galanin on adrenaline- and noradrenaline-induced increased oxytocin secretion in rat neurohypophyseal cell cultures",
abstract = "The effects of the interactions between the 29 amino acid-containing peptide galanin and adrenaline or noradrenaline on the secretion of oxytocin were studied in 13- to 14-day cultures of isolated rat neurohypophyseal tissue. The α-receptor antagonist corynanthine blocked the adrenaline-induced increase of oxytocin secretion. When the β-receptor antagonist propranolol was added before the noradrenaline treatment, the antagonist prevented the noradrenaline-induced enhancement of oxytocin release. Following the addition of galanin, the extent of oxytocin secretion into the supernatant medium decreased. Adrenaline and noradrenaline treatments increased the oxytocin level. Preincubation with galanin reduced the adrenaline- and noradrenaline-induced oxytocin level elevations. The blocking effect of galanin was prevented by previous treatment with the galanin receptor antagonist galantid (M15). When adrenaline or noradrenaline treatment was applied before galanin addition, the oxytocin secretion remained enhanced. The present results indicate that the changes in oxytocin secretion induced by the adrenergic system can be directly influenced by the galaninergic system. The interactions between the adrenergic and galaninergic systems from the aspect of oxytocin secretion can occur at the level of the posterior pituitary, independently of the hypothalamus.",
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author = "Marianna Rad{\'a}cs and A. Moln{\'a}r and F. L{\'a}szl{\'o} and C. Varga and Ferenc L{\'a}sel{\'o} and M. G{\'a}lfi",
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AU - Radács, Marianna

AU - Molnár, A.

AU - László, F.

AU - Varga, C.

AU - Láseló, Ferenc

AU - Gálfi, M.

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AB - The effects of the interactions between the 29 amino acid-containing peptide galanin and adrenaline or noradrenaline on the secretion of oxytocin were studied in 13- to 14-day cultures of isolated rat neurohypophyseal tissue. The α-receptor antagonist corynanthine blocked the adrenaline-induced increase of oxytocin secretion. When the β-receptor antagonist propranolol was added before the noradrenaline treatment, the antagonist prevented the noradrenaline-induced enhancement of oxytocin release. Following the addition of galanin, the extent of oxytocin secretion into the supernatant medium decreased. Adrenaline and noradrenaline treatments increased the oxytocin level. Preincubation with galanin reduced the adrenaline- and noradrenaline-induced oxytocin level elevations. The blocking effect of galanin was prevented by previous treatment with the galanin receptor antagonist galantid (M15). When adrenaline or noradrenaline treatment was applied before galanin addition, the oxytocin secretion remained enhanced. The present results indicate that the changes in oxytocin secretion induced by the adrenergic system can be directly influenced by the galaninergic system. The interactions between the adrenergic and galaninergic systems from the aspect of oxytocin secretion can occur at the level of the posterior pituitary, independently of the hypothalamus.

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KW - Noradrenaline

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