Inhibitor sensitivity of human serum and vascular semicarbazide-sensitive amine oxidases

Zs Meszaros, A. Csanyi, G. Vallus, T. Szombathy, I. Karádi, K. Magyar

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Recent data suggest that elevated serum semicarbazide-sensitive amine oxidase activity (SSAO) may cause endothelial injury. Formation of cytotoxic metabolites (especially formaldehyde) and increased oxidative stress might lead to initiation or progression of atherosclerosis. Effective and selective inhibitors of human SSAO might exert cytoprotective effect on endothelial cells. To compare the inhibitor sensitivity of human serum and vascular tissue SSAO enzyme, the inhibitory effect of semicarbazide and MDL 72974A was investigated. Serum and vascular SSAO activity has been determined using 14C-benzylamine as a substrate. The IC50 values of semicarbazide were estimated to be 5x10-3M and 5x10-4M for SSAO from human serum and saphenous vein, respectively. MDL 72974A amine oxidase inhibitor was more than thousand times more effective than semicarbazide. The IC50 values were 10-7M and 10-8M for SSAO from human serum and saphenous vein, respectively. This finding supports the hypothesis that soluble and membrane-bound vascular SSAO enzymes might have similar structure.

Original languageEnglish
Pages (from-to)215-223
Number of pages9
JournalNeurobiology
Volume8
Issue number2
Publication statusPublished - 2000

Fingerprint

Amine Oxidase (Copper-Containing)
Blood Vessels
Serum
Saphenous Vein
Human Activities
Inhibitory Concentration 50
Enzymes
Formaldehyde
Amines
Atherosclerosis
Oxidoreductases
Oxidative Stress
Endothelial Cells
Membranes
Wounds and Injuries

Keywords

  • Amine oxidase
  • Semicarbazide
  • Serum
  • Smooth muscle

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Inhibitor sensitivity of human serum and vascular semicarbazide-sensitive amine oxidases. / Meszaros, Zs; Csanyi, A.; Vallus, G.; Szombathy, T.; Karádi, I.; Magyar, K.

In: Neurobiology, Vol. 8, No. 2, 2000, p. 215-223.

Research output: Contribution to journalArticle

Meszaros, Z, Csanyi, A, Vallus, G, Szombathy, T, Karádi, I & Magyar, K 2000, 'Inhibitor sensitivity of human serum and vascular semicarbazide-sensitive amine oxidases', Neurobiology, vol. 8, no. 2, pp. 215-223.
Meszaros, Zs ; Csanyi, A. ; Vallus, G. ; Szombathy, T. ; Karádi, I. ; Magyar, K. / Inhibitor sensitivity of human serum and vascular semicarbazide-sensitive amine oxidases. In: Neurobiology. 2000 ; Vol. 8, No. 2. pp. 215-223.
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