Inhibition of the formation of amyloid-like fibrils with spices, especially cloves

M. Kotormán, Alexandra Varga, Phanindra Babu Kasi, János Nemcsók

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

During the study of inhibition of amyloid fibril formation, α-chymotrypsin protein was developed in 55% ethanol at pH 7.0. We investigated the inhibitory effect of different spices on amyloid fibril formation using turbidity measurements and Congo red binding assays. We found that all spices except the black pepper and caraway seed prevented fibril formation. The highest inhibition was measured with the clove, which reduced the amount of aggregates by 90%. We studied the inhibitory effect of the cloves at different concentrations on aggregation, it was found that the inhibitory activity of clove is dependent on concentration. We have measured the total phenolic content of the spice extracts too. Based on all these findings we have come to the following conclusion: Our results indicate that spices can contain other compounds too - not only phenolic compounds - which influence the formation of amyloid fibrils, and the effectiveness of various phenolic compounds are different.

Original languageEnglish
Pages (from-to)385-394
Number of pages10
JournalActa Biologica Hungarica
Volume69
Issue number4
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

Syzygium
spice
Spices
Amyloid
phenolic compound
Congo Red
Piper nigrum
Carum
Chymotrypsin
Turbidity
Seed
Assays
Ethanol
Agglomeration
turbidity
ethanol
Seeds
assay
seed
protein

Keywords

  • Amyloid fibrils
  • Clove
  • Polyphenols
  • Spice
  • α-Chymotrypsin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

Inhibition of the formation of amyloid-like fibrils with spices, especially cloves. / Kotormán, M.; Varga, Alexandra; Kasi, Phanindra Babu; Nemcsók, János.

In: Acta Biologica Hungarica, Vol. 69, No. 4, 01.12.2018, p. 385-394.

Research output: Contribution to journalArticle

Kotormán, M. ; Varga, Alexandra ; Kasi, Phanindra Babu ; Nemcsók, János. / Inhibition of the formation of amyloid-like fibrils with spices, especially cloves. In: Acta Biologica Hungarica. 2018 ; Vol. 69, No. 4. pp. 385-394.
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