Inhibition of the formation of amyloid-like fibrils using herbal extracts

Márta KotorMán, Zita Kelemen, Phanindra Ba Bu Kasi, János Nemcsók

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3 Citations (Scopus)

Abstract

We tested the amyloid fibril formation inhibitory effect of seven teas diluted in 55% ethanol at pH 7.0 at a protein concentration of 0.15 mg/ml α-chymotrypsin. In the experiments we investigated the formation and inhibition of amyloid fibrils by turbidity measurements, aggregation kinetics experiments and Congo red binding assay. The results suggest that the different teas effectively inhibit the formation of amyloid-like fibrils. The two most potent inhibitors were peppermint and melilot, extracts which almost completely inhibited the formation of aggregates in 5-fold dilution. The inhibitory effect on the aggregation formation of melilot and peppermint extracts was concentration dependant. The extent of inhibition was found to be proportional with the total concentration of phenolic compounds.

Original languageEnglish
Pages (from-to)125-134
Number of pages10
JournalActa biologica Hungarica
Volume69
Issue number2
DOIs
Publication statusPublished - Jun 2018

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Keywords

  • Amyloid fibrils
  • Melilot
  • Peppermint
  • Polyphenols
  • Α-Chymotrypsin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

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