Inhibition of the CD3-mediated calcium signal by protein kinase C activators in human T (Jurkat) lymphoblastoid cells

A. Tordai, B. Sarkadi, Gy Görög, G. Gárdos

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The changes in the cytoplasmic free calcium concentrations (Cai) were investigated in human T (Jurkat) lymphoblastoid cells, loaded with the calcium-sensitive fluorescent dye Indo-1. A rapid increase in Cai could be evoked by monoclonal antibodies (mAb) directed against the CD3 antigen, as well as by the mitogenic lectin concanavalin A (conA). The protein kinase C (pKC) activators TPA and DiC8 did not increase Cai, but the addition of any of these two compounds prior to mAb eliminated the mAb-induced calcium signal. In contrast, the calcium signal evoked by ConA was not blocked by TPA or DiC8. These results suggest that the CD3-dependent calcium signal is selectively down-regulated by the activation of pKC.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalImmunology letters
Volume20
Issue number1
DOIs
Publication statusPublished - Jan 15 1989

Keywords

  • 1,2-dioctanoyl glycerol
  • CD3 antigen
  • Ca
  • Calcium signal
  • ConA
  • DAG
  • DC8
  • IL-2
  • IP
  • Interleukin 2
  • Jurkat
  • Phorbol ester
  • Protein kinase C
  • T cells
  • T-cell receptor
  • TCR
  • TPA
  • aCD3
  • aTCR
  • concanavalin A
  • cytoplasmic free Ca concentration
  • diacyl-glycerol
  • iTPA
  • inositol-triphosphate
  • mAb
  • monoclonal antibody
  • monoclonal antibody against CD3 antigen
  • monoclonal antibody against the T-cell receptor
  • pKC
  • phorbol 12,13-dibutyrate
  • phorbol 12-myristate 13-acetate (PMA)
  • protein kinase C

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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