Inhibition of small intestinal colonization of enterotoxigenic Escherichia coly by Streptococcus faecium M74 in pigs

T. C. Ushe, B. Nagy

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Citations (Scopus)

Abstract

Colonizing and anti E. coli activity of S. faecium M74 was tested by oral inoculation of cesarean derived, colostrum deprived piglets with Streptococcus faecium M74 and subsequently with a heat stable enterotoxin (ST) producing E. coli (O101 : K30 : K99 : NM). Enterotoxin neutralization and co-culture studies were also performed in vitro. Bacterial counts in 10 cm ileal segments, fluorescein antibody stained cryostat sections, as well as 0.5 μm sections from the ilea of the experimental pigs taken 16 hours post exposure to enterotoxic E. coli (ETEC) all indicated that S. faecium M74 could not colonize the ileum of the newborn pigs, in a single high (7 x 108 - 3 x 1010) dose either in TSB or in milk suspension, in contrast to the ETEC. However, S. faecium given in milk suspension resulted in a marked decrease in ileal colonization of ETEC and in weight loss of piglets. In vitro expriments indicated that neither extracellular nor cell-bound products of S. faecium M74 could neutralise ST, but there was a significant reduction of pH in the TSB cultures of S. faecium that was accompanied by a reduction in ETEC counts of the mixed cultures.

Original languageEnglish
Title of host publicationZentralblatt fur Bakteriologie Mikrobiologie und Hygiene - Abt. 1 Orig. B. Hygiene
Pages374-382
Number of pages9
Volume181
Edition3-5
Publication statusPublished - 1985

Fingerprint

Escherichia
Enterococcus faecium
Swine
Escherichia coli
Ileum
Suspensions
Milk
Colostrum
Bacterial Load
Enterotoxins
Coculture Techniques
Fluorescein
Weight Loss
Hot Temperature
Antibodies

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Ushe, T. C., & Nagy, B. (1985). Inhibition of small intestinal colonization of enterotoxigenic Escherichia coly by Streptococcus faecium M74 in pigs. In Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene - Abt. 1 Orig. B. Hygiene (3-5 ed., Vol. 181, pp. 374-382)

Inhibition of small intestinal colonization of enterotoxigenic Escherichia coly by Streptococcus faecium M74 in pigs. / Ushe, T. C.; Nagy, B.

Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene - Abt. 1 Orig. B. Hygiene. Vol. 181 3-5. ed. 1985. p. 374-382.

Research output: Chapter in Book/Report/Conference proceedingChapter

Ushe, TC & Nagy, B 1985, Inhibition of small intestinal colonization of enterotoxigenic Escherichia coly by Streptococcus faecium M74 in pigs. in Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene - Abt. 1 Orig. B. Hygiene. 3-5 edn, vol. 181, pp. 374-382.
Ushe TC, Nagy B. Inhibition of small intestinal colonization of enterotoxigenic Escherichia coly by Streptococcus faecium M74 in pigs. In Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene - Abt. 1 Orig. B. Hygiene. 3-5 ed. Vol. 181. 1985. p. 374-382
Ushe, T. C. ; Nagy, B. / Inhibition of small intestinal colonization of enterotoxigenic Escherichia coly by Streptococcus faecium M74 in pigs. Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene - Abt. 1 Orig. B. Hygiene. Vol. 181 3-5. ed. 1985. pp. 374-382
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