An in vitro system containing a rabbit serum mediumadapted subline of L5178Y cells and rabbit antiserum was used to study the effects of antibodies, in the absence of complement, on cells in culture. Heat-inactivated antiserurn inhibits cell growth and metabolism. These actions occur slowly. RNA synthesis is inhibited after 4 h, but the effects on the rates of protein and DNA synthesis become demonstrable only after 6 to 8, and 8 h, respectively. Maximal inhibition can be obtained after 24 to 48 h. These effects together with the apparent involvement of energy generating systems suggest that some indirect mechanism, triggered as a result of primary antigen-antibody reactions on the cell surface, is responsible.
ASJC Scopus subject areas
- Cell Biology