Inhibition of pituitary-gonadal axis in mice by long-term administration of D-Trp-6-LHRH microcapsules

L. Bokser, A. Zalatnai, A. V. Schally

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Abstract

Female mice were injected, every 30 days for 5 months, with a long-acting formulation of microcapsules liberating 2.5 μg D-Trp-6-LHRH/day. The control group was injected with vehicle only. At 30 days after the last injection mice were killed, ovaries, uteri and adrenals were weighed and fixed in formalin for histological studies. LH and oestradiol concentrations were measured by RIA. In the D-Trp-6-LHRH-treated groups, the weights of the ovaries and uterus (P < 0.01 and P < 0.05, respectively), and LH and oestradiol values (P < 0.02 and P < 0.01, respectively) were reduced compared to controls. Histologically, the ovaries contained a large number of degenerated, atretic follicles, and corpora lutea had almost completely disappeared. These results indicate, contrary to the prevailing opinion, that mice are sensitive to inhibitory effects of LHRH agonists and that a suppression of the pituitary-gonadal axis can be obtained with long-term administration of D-Trp-6-LHRH microcapsules.

Original languageEnglish
Pages (from-to)569-574
Number of pages6
JournalJournal of Reproduction and Fertility
Volume85
Issue number2
DOIs
Publication statusPublished - Jan 1 1989

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ASJC Scopus subject areas

  • Physiology
  • Embryology
  • Molecular Biology
  • Obstetrics and Gynaecology
  • Developmental Biology

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