Inhibition of Na+-K+-dependent atp-ase from mucosa of human small intestine by butyl-biguanide

T. Beró, Petz, J. Kutas, Gy Mózsik

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Abstract

The membrane ATP-ase was prepared from mucosa of human small intestine with differential centrifugation and treated with 2 M NaJ. The effect of butyl-biguanide, which inhibits the active transport (absorption) of glucose from the small intestine in man and also in animals, has been studied in relation to Mg++-dependent, total (Na+-K+- dependent and Mg++-dependent) and Na+-K+-dependent ATP-ase activities exclusively. A significant inhibition of total and Na+-K+-dependent ATP-ase was caused by butylbiguanide, while no significant stimulation of Mg++-dependent ATP-ase activity was obtained. The possible role of Na+-K+-dependent ATP-ase activity in the glucose absorption from small intestine, on the one hand, and the inhibition by butyl-biguanide of glucose absorption, on the other hand, have been discussed.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalPharmacology
Volume11
Issue number4
DOIs
Publication statusPublished - Jan 1 1974

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Keywords

  • Active transport
  • Glucose absorption
  • Human small intestine
  • Na-K-dependent ATP-ase

ASJC Scopus subject areas

  • Pharmacology

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