Inhibition of moloney murine leukemia virus reverse transcriptase by partially 5-thiolated polyuridylic acid

Szilvia Tőkés, Janos Aradi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Partially 5-thiolated polyuridylic acid (poly(U 60 hs 5 U 40 )) is shown to be a potent inhibitor of Moloney murine leukemia virus Reverse Transcriptase (M-MuLV RT). The pattern of this inhibition is competitive when either poly(A) · (dT) 16 or poly(C) · (dG) 16 as template-primer (variable substrate) are used, suggesting that the free enzyme interacts with the modified polynucleotide. K m and K i values of 25 μM and 11 nM, respectively were obtained in the presence of poly(A) · (dT) 16 . The K i value determined in the presence of poly(C) · (dG) 16 was 31 nM (K m = 22 μM). Inhibitory activities of the 5-thiolated oligouridylic acids, prepared from the polymer, depend on the chain-length. While the 30-mer showed the same activity as the intact polynucleotide, shorter oligonucleotides proved to be less active.

Original languageEnglish
Pages (from-to)115-120
Number of pages6
JournalBBA - Gene Structure and Expression
Volume1261
Issue number1
DOIs
Publication statusPublished - Mar 14 1995

Keywords

  • Inhibition
  • Modified polynucleotide
  • Reverse transcriptase

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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