Inhibition of migration of MDA-MB-231 cells by methyl-3,5-diiodo-4-(4′-methoxyphenoxy) benzoate (DIME)

Kalman G. Buki, Eva Kirsten, Pal I. Bauer, Charles A. Vidair, Albert Kun, Jerome Mendeleyev, Ernest Kun

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Abstract

The GTPase activity of purified dimeric tubulin (α+β) at 5 μM was insensitive to methyl-3,5-diiodo-4-(4′-methoxyphenoxy) benzoate (DIME), in contrast to nocodazole which activated GTPase. Cellular motility of MDA-MB-231 (human mammary cancer) cells migrating through 12-μm pores was inhibited by DIME similar to nocodazole in a drug concentration- and DIME structure-dependent manner. An increase of cytoplasmic ATPase activity of DIME-treated cells without a decrease in ATP contents of intact cells suggests that DIME may also influence additional as yet unidentified ATP-dependent system(s) probably also involved in cell motility. These results show that DIME not only arrests cells in M phase but also inhibits cell motility in interphase. However the cellular mode of action of DIME is different from the action of other toxic tubulin-targeted drugs, despite the fact that DIME in a concentration-dependent manner disrupts microtubule structures in intact cells.

Original languageEnglish
Pages (from-to)1247-1250
Number of pages4
JournalInternational journal of oncology
Volume11
Issue number6
Publication statusPublished - Dec 1 1997

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Keywords

  • 5-diiodo-4-(4′-methoxyphenoxy) benzoate
  • Methyl-3
  • Migration of cancer cells
  • Tubulin

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Buki, K. G., Kirsten, E., Bauer, P. I., Vidair, C. A., Kun, A., Mendeleyev, J., & Kun, E. (1997). Inhibition of migration of MDA-MB-231 cells by methyl-3,5-diiodo-4-(4′-methoxyphenoxy) benzoate (DIME). International journal of oncology, 11(6), 1247-1250.