Inhibition of migration of MDA-MB-231 cells by methyl-3,5-diiodo-4-(4'- methoxyphenoxy) benzoate (DIME)

K. G. Buki, E. Kirsten, P. Bauer, C. A. Vidair, A. Kun, J. Mendeleyev, E. Kun

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The GTPase activity of purified dimeric tubulin (α+β) at 5 μM was insensitive to methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME), in contrast to nocodazole which activated GTPase. Cellular motility of MDA-MB- 231 (human mammary cancer) celts migrating through 12-μm pores was inhibited by DIME similar to nocodazole in a drug concentration- and DIME structure- dependent manner. An increase of cytoplasmic ATPase activity of DIME-treated cells without a decrease in ATP contents of intact cells suggests that DIME may also influence additional as yet unidentified ATP-dependent system(s) probably also involved in cell motility. These results show that DIME not only arrests cells in M phase but also inhibits cell motility in interphase. However the cellular mode of action of DIME is different from the action of other toxic tubulin-targeted drugs, despite the fact that DIME in a concentration-dependent manner disrupts microtubule structures in intact cells.

Original languageEnglish
Pages (from-to)1247-1250
Number of pages4
JournalInternational Journal of Oncology
Volume11
Issue number6
Publication statusPublished - 1997

Fingerprint

Nocodazole
GTP Phosphohydrolases
Tubulin
Cell Movement
Adenosine Triphosphate
Toxic Actions
Interphase
Microtubules
Pharmaceutical Preparations
Cell Division
Adenosine Triphosphatases
Breast Neoplasms
methyl 3,5-diiodo-4-(4'-methoxyphenoxy)benzoate

Keywords

  • Methyl-3,5-diiodo-4-(4'- methoxyphenoxy) benzoate
  • Migration of cancer cells
  • Tubulin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Buki, K. G., Kirsten, E., Bauer, P., Vidair, C. A., Kun, A., Mendeleyev, J., & Kun, E. (1997). Inhibition of migration of MDA-MB-231 cells by methyl-3,5-diiodo-4-(4'- methoxyphenoxy) benzoate (DIME). International Journal of Oncology, 11(6), 1247-1250.

Inhibition of migration of MDA-MB-231 cells by methyl-3,5-diiodo-4-(4'- methoxyphenoxy) benzoate (DIME). / Buki, K. G.; Kirsten, E.; Bauer, P.; Vidair, C. A.; Kun, A.; Mendeleyev, J.; Kun, E.

In: International Journal of Oncology, Vol. 11, No. 6, 1997, p. 1247-1250.

Research output: Contribution to journalArticle

Buki, KG, Kirsten, E, Bauer, P, Vidair, CA, Kun, A, Mendeleyev, J & Kun, E 1997, 'Inhibition of migration of MDA-MB-231 cells by methyl-3,5-diiodo-4-(4'- methoxyphenoxy) benzoate (DIME)', International Journal of Oncology, vol. 11, no. 6, pp. 1247-1250.
Buki, K. G. ; Kirsten, E. ; Bauer, P. ; Vidair, C. A. ; Kun, A. ; Mendeleyev, J. ; Kun, E. / Inhibition of migration of MDA-MB-231 cells by methyl-3,5-diiodo-4-(4'- methoxyphenoxy) benzoate (DIME). In: International Journal of Oncology. 1997 ; Vol. 11, No. 6. pp. 1247-1250.
@article{5dd7ddf7696c4390a347ef1b11283cff,
title = "Inhibition of migration of MDA-MB-231 cells by methyl-3,5-diiodo-4-(4'- methoxyphenoxy) benzoate (DIME)",
abstract = "The GTPase activity of purified dimeric tubulin (α+β) at 5 μM was insensitive to methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME), in contrast to nocodazole which activated GTPase. Cellular motility of MDA-MB- 231 (human mammary cancer) celts migrating through 12-μm pores was inhibited by DIME similar to nocodazole in a drug concentration- and DIME structure- dependent manner. An increase of cytoplasmic ATPase activity of DIME-treated cells without a decrease in ATP contents of intact cells suggests that DIME may also influence additional as yet unidentified ATP-dependent system(s) probably also involved in cell motility. These results show that DIME not only arrests cells in M phase but also inhibits cell motility in interphase. However the cellular mode of action of DIME is different from the action of other toxic tubulin-targeted drugs, despite the fact that DIME in a concentration-dependent manner disrupts microtubule structures in intact cells.",
keywords = "Methyl-3,5-diiodo-4-(4'- methoxyphenoxy) benzoate, Migration of cancer cells, Tubulin",
author = "Buki, {K. G.} and E. Kirsten and P. Bauer and Vidair, {C. A.} and A. Kun and J. Mendeleyev and E. Kun",
year = "1997",
language = "English",
volume = "11",
pages = "1247--1250",
journal = "International Journal of Oncology",
issn = "1019-6439",
publisher = "Spandidos Publications",
number = "6",

}

TY - JOUR

T1 - Inhibition of migration of MDA-MB-231 cells by methyl-3,5-diiodo-4-(4'- methoxyphenoxy) benzoate (DIME)

AU - Buki, K. G.

AU - Kirsten, E.

AU - Bauer, P.

AU - Vidair, C. A.

AU - Kun, A.

AU - Mendeleyev, J.

AU - Kun, E.

PY - 1997

Y1 - 1997

N2 - The GTPase activity of purified dimeric tubulin (α+β) at 5 μM was insensitive to methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME), in contrast to nocodazole which activated GTPase. Cellular motility of MDA-MB- 231 (human mammary cancer) celts migrating through 12-μm pores was inhibited by DIME similar to nocodazole in a drug concentration- and DIME structure- dependent manner. An increase of cytoplasmic ATPase activity of DIME-treated cells without a decrease in ATP contents of intact cells suggests that DIME may also influence additional as yet unidentified ATP-dependent system(s) probably also involved in cell motility. These results show that DIME not only arrests cells in M phase but also inhibits cell motility in interphase. However the cellular mode of action of DIME is different from the action of other toxic tubulin-targeted drugs, despite the fact that DIME in a concentration-dependent manner disrupts microtubule structures in intact cells.

AB - The GTPase activity of purified dimeric tubulin (α+β) at 5 μM was insensitive to methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME), in contrast to nocodazole which activated GTPase. Cellular motility of MDA-MB- 231 (human mammary cancer) celts migrating through 12-μm pores was inhibited by DIME similar to nocodazole in a drug concentration- and DIME structure- dependent manner. An increase of cytoplasmic ATPase activity of DIME-treated cells without a decrease in ATP contents of intact cells suggests that DIME may also influence additional as yet unidentified ATP-dependent system(s) probably also involved in cell motility. These results show that DIME not only arrests cells in M phase but also inhibits cell motility in interphase. However the cellular mode of action of DIME is different from the action of other toxic tubulin-targeted drugs, despite the fact that DIME in a concentration-dependent manner disrupts microtubule structures in intact cells.

KW - Methyl-3,5-diiodo-4-(4'- methoxyphenoxy) benzoate

KW - Migration of cancer cells

KW - Tubulin

UR - http://www.scopus.com/inward/record.url?scp=0030815295&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030815295&partnerID=8YFLogxK

M3 - Article

C2 - 21528330

AN - SCOPUS:0030815295

VL - 11

SP - 1247

EP - 1250

JO - International Journal of Oncology

JF - International Journal of Oncology

SN - 1019-6439

IS - 6

ER -