Inhibition of IgE production by epsilon (ε) chain-specific antisense oligonucleotides (AOs) studied on human myeloma cell line U266 and peripheral blood mononuclear cells of a patient with hypereosinophilia

Béla Fekete, János Sági, Attila Szemzõ, László Kovács, Katalin Pálóczi, Valéria L. Varga, Klára Tamássy, András Falus

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Based on cDNA sequence data ε chain-specific antisense oligonucleotides were synthesized and checked on in vitro IgE production. Using peripheral blood cells from a hypereosinophilic patient and a human IgE myeloma cell line, U266, marked reduction of in vitro IgE production measured by PRIST was observed. The effect of ε antisenses proved to be isotype specific since IgG production by both peripheral blood cells and a lymphoma cell line, CESS, was not affected. Moreover, the expression of other markers on U266 (interleukin- 6 receptor and gp 130) were not influenced by ε-specific antisense oligonucleotides.

Original languageEnglish
Pages (from-to)181-190
Number of pages10
JournalImmunology letters
Issue number3
Publication statusPublished - Aug 1 1997



  • Antisense oligonucleotide
  • IgE production

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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