Inhibition of IgE-mediated triggering of mast cells by complement- derived peptides interacting with the FcεRI

Anna Erdei, Gábor K. Tóth, Márton Andrásfalvy, János Matkó, László Bene, Zsuzsa Bajtay, Alexander Ischenko, Xu Rong, Israel Pecht

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Mucosal type mast cells, in contrast to the serosal type ones, do not respond to cationic agents, or to the complement-derived peptides C3a and C5a [1]. Earlier we have found that while C3a does not activate the rat mucosal type mast cells (line RBL-2H3), it strongly inhibits the IgE-mediated triggering of these cells, by interfering with the FcεRI-initiated signaling pathway [2]. In the present study we further investigated the mechanism of this process. It is shown, that C3a interacts with the β-chain of the FcεRI complex. Binding of the complement peptide to the cells apparently causes a decrease in the proximity of the IgE-binding FcεRI. Investigating certain sequences of C3a we found that the inhibition is caused by the C-terminal sequences of the complement-peptide, ranging from positions 56 to 77 and also by a shorter sequence, ranging from positions 56 to 64. The inhibitory effect of these peptides was observed both in the case of RBL-2H3 cells and mouse bone marrow derived mast cells.

Original languageEnglish
Pages (from-to)79-82
Number of pages4
JournalImmunology letters
Volume68
Issue number1
DOIs
Publication statusPublished - May 3 1999

Keywords

  • Bone marrow derived mast cells
  • C3a
  • Inhibition of IgE- mediated triggering
  • RBL-2H3 cells
  • β-chain of FcεRI

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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