Inhibition of hepatic glucose 6-phosphatase system by the green tea flavanol epigallocatechin gallate

Miklós Csala, Éva Margittai, Silvia Senesi, Alessandra Gamberucci, Gábor Bánhegyi, József Mandl, Angelo Benedetti

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Effect of 5-100 μM epigallocatechin gallate (EGCG) on hepatic glucose 6-phosphatase (G6Pase) system was investigated. EGCG inhibited G6Pase in intact but not in permeabilized rat liver microsomes, suggesting the interference with the transport. However, EGCG did not hinder microsomal glucose 6-phosphate (G6P) uptake. Instead, it increased the accumulation of radioactivity after the addition of [14C]G6P, presumably due to a slower release of [14C]glucose, the product of luminal hydrolysis. Indeed, EGCG was found to inhibit microsomal glucose efflux. Since G6Pase activity is depressed by glucose in a concentration-dependent manner, we concluded that EGCG inhibits G6Pase through an elevated luminal glucose level.

Original languageEnglish
Pages (from-to)1693-1698
Number of pages6
JournalFEBS letters
Volume581
Issue number8
DOIs
Publication statusPublished - Apr 17 2007

Keywords

  • Catechin
  • Endoplasmic reticulum
  • Epigallocatechin gallate
  • Glucose
  • Green tea
  • Transport

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Inhibition of hepatic glucose 6-phosphatase system by the green tea flavanol epigallocatechin gallate'. Together they form a unique fingerprint.

  • Cite this