Inhibition of experimental gingivitis in beagle dogs with topical mercaptoalkylguanidines

David W. Paquette, Adam Rosenberg, Zs. Lohinai, Gary J. Southan, Ray C. Williams, Steven Offenbacher, Csaba Szabò

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Nitric oxide is a free radical produced in host tissues by constitutive and inducible forms of the enzyme nitric oxide synthase. Nitric oxide plays physiological roles, but it is also involved in the pathophysiology of several inflammatory conditions, including arthritis, ulcerative colitis, and circulatory shock. Local increases in inducible nitric oxide synthase (iNOS) and reactive nitrogen products have also been demonstrated in humans and animals with periodontal disease. This masked, randomized, placebo-controlled preclinical investigation examined the effect of two mercaptoalkylguanidines, mercaptoethylguanidine (MEG) and guanidinoethyldisulfide (GED), which are iNOS inhibitors and reactive nitrogen scavenging compounds, on the development of experimental gingivitis in beagle dogs. Methods: Fifteen female, 1-year-old beagles first completed a 2-week dose-escalation experiment during which a maximum tolerated dose was determined for MEG and GED gels. Thereafter, all animals were brought to optimal gingival health by mechanical scaling, followed by rigorous daily toothbrushing over a 4-week washout period. Experimental gingivitis was then induced, with cessation of plaque control and institution of a soft diet over 8 weeks. Beagles randomly received 0.3% MEG, 0.3% GED, or placebo (vehicle) gels, topically applied twice daily to premolar teeth. Gingival inflammation, bleeding tendency, and supragingival plaque were clinically measured at baseline and at 2, 3, 4, 6, and 8 weeks. Comparisons among groups and between group pairs (active versus placebo) were made using Kruskal-Wallis tests. Results: From baseline to day 7, all groups expressed similar indices. Thereafter, significant and time-dependent increases in the plaque index (PI), gingival index (GI), and percentage of bleeding on probing (%BOP) were observed in placebo-treated beagles. Mean GI scores for beagles treated with GED or MEG gels remained at or below baseline levels for the entire treatment period. At weeks 2, 3, 4, and 8, GI scores were significantly lower for MEG and GED groups compared to the placebo group (P

Original languageEnglish
Pages (from-to)385-391
Number of pages7
JournalJournal of Periodontology
Volume77
Issue number3
DOIs
Publication statusPublished - Mar 2006

Fingerprint

Gingivitis
Periodontal Index
Placebos
Dogs
Gels
Nitric Oxide Synthase Type II
Nitric Oxide
Hemorrhage
Nitrogen Compounds
Toothbrushing
Maximum Tolerated Dose
Bicuspid
Periodontal Diseases
Ulcerative Colitis
Nitric Oxide Synthase
Arthritis
Free Radicals
Shock
Tooth
Nitrogen

Keywords

  • Dogs
  • Gingivitis
  • Inducible nitric oxide synthase
  • Periodontal disease
  • Reactive nitrogen species

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Paquette, D. W., Rosenberg, A., Lohinai, Z., Southan, G. J., Williams, R. C., Offenbacher, S., & Szabò, C. (2006). Inhibition of experimental gingivitis in beagle dogs with topical mercaptoalkylguanidines. Journal of Periodontology, 77(3), 385-391. https://doi.org/10.1902/jop.2006.050049

Inhibition of experimental gingivitis in beagle dogs with topical mercaptoalkylguanidines. / Paquette, David W.; Rosenberg, Adam; Lohinai, Zs.; Southan, Gary J.; Williams, Ray C.; Offenbacher, Steven; Szabò, Csaba.

In: Journal of Periodontology, Vol. 77, No. 3, 03.2006, p. 385-391.

Research output: Contribution to journalArticle

Paquette, DW, Rosenberg, A, Lohinai, Z, Southan, GJ, Williams, RC, Offenbacher, S & Szabò, C 2006, 'Inhibition of experimental gingivitis in beagle dogs with topical mercaptoalkylguanidines', Journal of Periodontology, vol. 77, no. 3, pp. 385-391. https://doi.org/10.1902/jop.2006.050049
Paquette, David W. ; Rosenberg, Adam ; Lohinai, Zs. ; Southan, Gary J. ; Williams, Ray C. ; Offenbacher, Steven ; Szabò, Csaba. / Inhibition of experimental gingivitis in beagle dogs with topical mercaptoalkylguanidines. In: Journal of Periodontology. 2006 ; Vol. 77, No. 3. pp. 385-391.
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