Inhibition of Colorado potato beetle larvae by a locust proteinase inhibitor peptide expressed in potato

Mihály Kondrák, János Kutas, Borbála Szenthe, A. Pátthy, Z. Bánfalvi, Miklós Nádasy, L. Gráf, Bence Asbóth

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The cDNA for a 73-mer peptide containing two locust serine proteinase inhibitors was cloned, fused to the constitutive CaMV35S promoter and introduced into potato by Agrobacterium-mediated transformation. From 23 independent transgenic lines, three with high mRNA level and proteinase inhibitory activity were propagated in vitro and transferred to pots. The peptide from the leaves was identified by its N-terminal sequence and by K i values against chymotrypsin and trypsin. Colorado potato beetle larvae reared on transgenic plants grew slightly but significantly more slowly than those on control plants. This supports the notion that expression of multifunctional proteinase inhibitors of insect origin might be a good strategy to improve insect resistance in plants.

Original languageEnglish
Pages (from-to)829-834
Number of pages6
JournalBiotechnology Letters
Volume27
Issue number12
DOIs
Publication statusPublished - Jun 2005

Fingerprint

Grasshoppers
Beetles
Solanum tuberosum
Peptides
Larva
Insects
Peptide Hydrolases
Agrobacterium
Serine Proteinase Inhibitors
Genetically Modified Plants
Complementary DNA
Messenger RNA
In Vitro Techniques
trypsin drug combination chymotrypsin

Keywords

  • Beetle resistance
  • Larval growth
  • Locust inhibitory peptide
  • Multifunctional inhibitor
  • Transgenic potato

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Inhibition of Colorado potato beetle larvae by a locust proteinase inhibitor peptide expressed in potato. / Kondrák, Mihály; Kutas, János; Szenthe, Borbála; Pátthy, A.; Bánfalvi, Z.; Nádasy, Miklós; Gráf, L.; Asbóth, Bence.

In: Biotechnology Letters, Vol. 27, No. 12, 06.2005, p. 829-834.

Research output: Contribution to journalArticle

Kondrák, Mihály ; Kutas, János ; Szenthe, Borbála ; Pátthy, A. ; Bánfalvi, Z. ; Nádasy, Miklós ; Gráf, L. ; Asbóth, Bence. / Inhibition of Colorado potato beetle larvae by a locust proteinase inhibitor peptide expressed in potato. In: Biotechnology Letters. 2005 ; Vol. 27, No. 12. pp. 829-834.
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