Inhibition of calcium spikes by gamma-amino-butyric acid in the neurons of Lymnaea stagnalis L.

S. S. Rubakhin, A. Szücs, V. N. Gurin, K. S. Rózsa

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Abstract

The modulation of action potentials (APs) and high-threshold calcium currents by gamma-aminobutyric acid (GABA) and GABAB receptor agonist baclofen was investigated in identified internally perfused neurons of Lymnaea stagnalis using current-clamp and whole-cell voltage-clamp techniques. GABA (5 x 10(-5) M) and baclofen (5 x 10(-5) M) reversibly reduced overshoot and afterhyperpolarization phase of the action potentials. Application of GABA or baclofen inhibited inward and outward voltage-gated ionic currents. GABA (10(-8)-10(-4) M) was found to suppress high-threshold calcium current in voltage- and dose-dependent manner. These effects were mimicked by baclofen (5 x 10(-5) M) but not by muscimol (5 x 10(-5) M). Inhibition of Ca(2+)-currents could not be prevented by pentylenetetrazole (5 x 10(-5) M) or with pertussis toxin (1 microgram/ml). These results provide evidences for the presence of GABAB receptors in the identified central neurons of Lymnaea stagnalis L.

Original languageEnglish
Pages (from-to)375-380
Number of pages6
JournalActa biologica Hungarica
Volume46
Issue number2-4
Publication statusPublished - 1995

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

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