Inhibition of calcium spikes by gamma-amino-butyric acid in the neurons of Lymnaea stagnalis L.

S. S. Rubakhin, A. Szűcs, V. N. Gurin, K. S. Rózsa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The modulation of action potentials (APs) and high-threshold calcium currents by gamma-aminobutyric acid (GABA) and GABAB receptor agonist baclofen was investigated in identified internally perfused neurons of Lymnaea stagnalis using current-clamp and whole-cell voltage-clamp techniques. GABA (5 x 10(-5) M) and baclofen (5 x 10(-5) M) reversibly reduced overshoot and afterhyperpolarization phase of the action potentials. Application of GABA or baclofen inhibited inward and outward voltage-gated ionic currents. GABA (10(-8)-10(-4) M) was found to suppress high-threshold calcium current in voltage- and dose-dependent manner. These effects were mimicked by baclofen (5 x 10(-5) M) but not by muscimol (5 x 10(-5) M). Inhibition of Ca(2+)-currents could not be prevented by pentylenetetrazole (5 x 10(-5) M) or with pertussis toxin (1 microgram/ml). These results provide evidences for the presence of GABAB receptors in the identified central neurons of Lymnaea stagnalis L.

Original languageEnglish
Pages (from-to)375-380
Number of pages6
JournalActa Biologica Hungarica
Volume46
Issue number2-4
Publication statusPublished - 1995

Fingerprint

Lymnaea
Baclofen
Calcium Signaling
Butyric Acid
Neurons
amino acid
calcium
gamma-Aminobutyric Acid
Calcium
acid
Clamping devices
Action Potentials
Electric potential
Muscimol
Pentylenetetrazole
GABA Receptors
Pertussis Toxin
Patch-Clamp Techniques
toxin
Modulation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

Inhibition of calcium spikes by gamma-amino-butyric acid in the neurons of Lymnaea stagnalis L. / Rubakhin, S. S.; Szűcs, A.; Gurin, V. N.; Rózsa, K. S.

In: Acta Biologica Hungarica, Vol. 46, No. 2-4, 1995, p. 375-380.

Research output: Contribution to journalArticle

Rubakhin, S. S. ; Szűcs, A. ; Gurin, V. N. ; Rózsa, K. S. / Inhibition of calcium spikes by gamma-amino-butyric acid in the neurons of Lymnaea stagnalis L. In: Acta Biologica Hungarica. 1995 ; Vol. 46, No. 2-4. pp. 375-380.
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