Inhibition of calcineurin activity and protection against cyclosporine A induced cytotoxicity by prednisolone sodium succinate in human peripheral mononuclear cells

S. Sipka, Kornélia Szücs, S. Szántó, Ildikó Kovács, Gabriella Lakos, P. Antal-Szalmás, G. Szegedi, P. Gergely

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4 Citations (Scopus)

Abstract

We have investigated the effects of prednisolone sodium succinate (Pss) and cyclosporin A (CSA), applied alone or concurrently, on the release of arachidonic acid (AA) (cytosolic phospholipase A2 (cPLA2) activity) and on the calcineurin (CN) activity of human peripheral blood mononuclear cells (PBMC). The cytotoxic damage to the cells treated by the drugs was estimated by the release of lactate dehydrogenase (LDH). We found that Pss (10-5 M) could inhibit the CN activity and higher concentrations (10-4 M) could decrease the cytotoxic damage caused by CSA (10-4 M) during their combined application. CSA had no specific effect on the release of AA from the cells. In the combined clinical use of glucocorticosteroids (GCS) and CSA, their additive inhibitory effect on CN activity and the protective membrane influence of GCS against the cytotoxicity of CSA may be beneficial. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)87-92
Number of pages6
JournalImmunopharmacology
Volume48
Issue number1
DOIs
Publication statusPublished - Jun 2000

Fingerprint

Calcineurin
Cyclosporine
Arachidonic Acid
Cytosolic Phospholipases A2
L-Lactate Dehydrogenase
Human Activities
Blood Cells
prednisolone hemisuccinate
Membranes
Pharmaceutical Preparations

Keywords

  • Arachidonic acid
  • Calcineurin
  • Cyclosporin A
  • Glucocorticosteroid

ASJC Scopus subject areas

  • Pharmacology

Cite this

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title = "Inhibition of calcineurin activity and protection against cyclosporine A induced cytotoxicity by prednisolone sodium succinate in human peripheral mononuclear cells",
abstract = "We have investigated the effects of prednisolone sodium succinate (Pss) and cyclosporin A (CSA), applied alone or concurrently, on the release of arachidonic acid (AA) (cytosolic phospholipase A2 (cPLA2) activity) and on the calcineurin (CN) activity of human peripheral blood mononuclear cells (PBMC). The cytotoxic damage to the cells treated by the drugs was estimated by the release of lactate dehydrogenase (LDH). We found that Pss (10-5 M) could inhibit the CN activity and higher concentrations (10-4 M) could decrease the cytotoxic damage caused by CSA (10-4 M) during their combined application. CSA had no specific effect on the release of AA from the cells. In the combined clinical use of glucocorticosteroids (GCS) and CSA, their additive inhibitory effect on CN activity and the protective membrane influence of GCS against the cytotoxicity of CSA may be beneficial. Copyright (C) 2000 Elsevier Science B.V.",
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author = "S. Sipka and Korn{\'e}lia Sz{\"u}cs and S. Sz{\'a}nt{\'o} and Ildik{\'o} Kov{\'a}cs and Gabriella Lakos and P. Antal-Szalm{\'a}s and G. Szegedi and P. Gergely",
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AU - Sipka, S.

AU - Szücs, Kornélia

AU - Szántó, S.

AU - Kovács, Ildikó

AU - Lakos, Gabriella

AU - Antal-Szalmás, P.

AU - Szegedi, G.

AU - Gergely, P.

PY - 2000/6

Y1 - 2000/6

N2 - We have investigated the effects of prednisolone sodium succinate (Pss) and cyclosporin A (CSA), applied alone or concurrently, on the release of arachidonic acid (AA) (cytosolic phospholipase A2 (cPLA2) activity) and on the calcineurin (CN) activity of human peripheral blood mononuclear cells (PBMC). The cytotoxic damage to the cells treated by the drugs was estimated by the release of lactate dehydrogenase (LDH). We found that Pss (10-5 M) could inhibit the CN activity and higher concentrations (10-4 M) could decrease the cytotoxic damage caused by CSA (10-4 M) during their combined application. CSA had no specific effect on the release of AA from the cells. In the combined clinical use of glucocorticosteroids (GCS) and CSA, their additive inhibitory effect on CN activity and the protective membrane influence of GCS against the cytotoxicity of CSA may be beneficial. Copyright (C) 2000 Elsevier Science B.V.

AB - We have investigated the effects of prednisolone sodium succinate (Pss) and cyclosporin A (CSA), applied alone or concurrently, on the release of arachidonic acid (AA) (cytosolic phospholipase A2 (cPLA2) activity) and on the calcineurin (CN) activity of human peripheral blood mononuclear cells (PBMC). The cytotoxic damage to the cells treated by the drugs was estimated by the release of lactate dehydrogenase (LDH). We found that Pss (10-5 M) could inhibit the CN activity and higher concentrations (10-4 M) could decrease the cytotoxic damage caused by CSA (10-4 M) during their combined application. CSA had no specific effect on the release of AA from the cells. In the combined clinical use of glucocorticosteroids (GCS) and CSA, their additive inhibitory effect on CN activity and the protective membrane influence of GCS against the cytotoxicity of CSA may be beneficial. Copyright (C) 2000 Elsevier Science B.V.

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