Inhibition of autophagic vacuole formation and protein degradation by amino acids in isolated hepatocytes

Attila L. Kovács, Bjørn Grinde, Per O. Seglen

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Abstract

An amino acid mixture, specifically developed to suppress endogenous protein degradation in isolated hepatocytes, inhibited lysosornal (propylamine-sensitive) protein degradation by 70-75% and reduced the cytoplasmic volume fraction of the autophagic/lysosomal compartment to a similar extent. Incubation with the amino acid mixture for 1 h reduced the subcompartment of early autophagic vacuoles by 95%. These results support the hypothesis that autophagy is the major route of delivery of endogenous proteins to the lysosomes, and that amino acids exert their regulatory function on protein degradation by controlling the sequestration step of autophagy.

Original languageEnglish
Pages (from-to)431-436
Number of pages6
JournalExperimental Cell Research
Volume133
Issue number2
DOIs
Publication statusPublished - Jun 1981

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ASJC Scopus subject areas

  • Cell Biology

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