Inhibition of arachidonic acid release from human peripheral mononuclear cells by heat shock treatment and geldanamycin

S. Szántó, P. Csermely, Ildikó Kovács, József Csongor, A. Illés, Gyula Bakó, G. Szegedi, S. Sipka

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The objective of this study was to investigate the effects of heat shock (HS) treatment and geldanamycin (GA) on the release of arachidonic acid (AA) from human peripheral blood mononuclear cells (PBMC), monocytes and lymphocytes. Mononuclear cells prepared from blood of healthy subjects were preincubated with 3H-AA. The release of 3H-AA incorporated into the membrane was studied after pretreatment of cells by HS (43°C, 1 h) and GA. The activation of AA producing enzymes was achieved by the addition of phorbol 12-myristate 13-acetate (PMA) or by the combination of PMA+calcium ionophore A-23187. Treatment of cells by HS inhibited the release of AA. Furthermore, the release of AA by PBMC was dose dependently inhibited by GA. The combination of treatments by HS and GA augmented the inhibition of AA release. The HS response involves a diminished release of AA from PBMC. The inhibitory effect of GA on the AA release is a new element in the antiinflammatory pharmacological ability of this drug.

Original languageEnglish
Pages (from-to)181-185
Number of pages5
JournalImmunology Letters
Volume83
Issue number3
DOIs
Publication statusPublished - Oct 1 2002

Fingerprint

Arachidonic Acid
Shock
Hot Temperature
Blood Cells
Therapeutics
Acetates
geldanamycin
Heat-Shock Response
Calcium Ionophores
Calcimycin
Monocytes
Healthy Volunteers
Anti-Inflammatory Agents
Pharmacology
Lymphocytes
Membranes
Enzymes
Pharmaceutical Preparations

Keywords

  • Arachidonic acid
  • Geldanamycin
  • Heat shock
  • Phospholipase A

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Inhibition of arachidonic acid release from human peripheral mononuclear cells by heat shock treatment and geldanamycin. / Szántó, S.; Csermely, P.; Kovács, Ildikó; Csongor, József; Illés, A.; Bakó, Gyula; Szegedi, G.; Sipka, S.

In: Immunology Letters, Vol. 83, No. 3, 01.10.2002, p. 181-185.

Research output: Contribution to journalArticle

@article{24a086b934dc4750892688c3c1b5525c,
title = "Inhibition of arachidonic acid release from human peripheral mononuclear cells by heat shock treatment and geldanamycin",
abstract = "The objective of this study was to investigate the effects of heat shock (HS) treatment and geldanamycin (GA) on the release of arachidonic acid (AA) from human peripheral blood mononuclear cells (PBMC), monocytes and lymphocytes. Mononuclear cells prepared from blood of healthy subjects were preincubated with 3H-AA. The release of 3H-AA incorporated into the membrane was studied after pretreatment of cells by HS (43°C, 1 h) and GA. The activation of AA producing enzymes was achieved by the addition of phorbol 12-myristate 13-acetate (PMA) or by the combination of PMA+calcium ionophore A-23187. Treatment of cells by HS inhibited the release of AA. Furthermore, the release of AA by PBMC was dose dependently inhibited by GA. The combination of treatments by HS and GA augmented the inhibition of AA release. The HS response involves a diminished release of AA from PBMC. The inhibitory effect of GA on the AA release is a new element in the antiinflammatory pharmacological ability of this drug.",
keywords = "Arachidonic acid, Geldanamycin, Heat shock, Phospholipase A",
author = "S. Sz{\'a}nt{\'o} and P. Csermely and Ildik{\'o} Kov{\'a}cs and J{\'o}zsef Csongor and A. Ill{\'e}s and Gyula Bak{\'o} and G. Szegedi and S. Sipka",
year = "2002",
month = "10",
day = "1",
doi = "10.1016/S0165-2478(02)00103-7",
language = "English",
volume = "83",
pages = "181--185",
journal = "Immunology Letters",
issn = "0165-2478",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Inhibition of arachidonic acid release from human peripheral mononuclear cells by heat shock treatment and geldanamycin

AU - Szántó, S.

AU - Csermely, P.

AU - Kovács, Ildikó

AU - Csongor, József

AU - Illés, A.

AU - Bakó, Gyula

AU - Szegedi, G.

AU - Sipka, S.

PY - 2002/10/1

Y1 - 2002/10/1

N2 - The objective of this study was to investigate the effects of heat shock (HS) treatment and geldanamycin (GA) on the release of arachidonic acid (AA) from human peripheral blood mononuclear cells (PBMC), monocytes and lymphocytes. Mononuclear cells prepared from blood of healthy subjects were preincubated with 3H-AA. The release of 3H-AA incorporated into the membrane was studied after pretreatment of cells by HS (43°C, 1 h) and GA. The activation of AA producing enzymes was achieved by the addition of phorbol 12-myristate 13-acetate (PMA) or by the combination of PMA+calcium ionophore A-23187. Treatment of cells by HS inhibited the release of AA. Furthermore, the release of AA by PBMC was dose dependently inhibited by GA. The combination of treatments by HS and GA augmented the inhibition of AA release. The HS response involves a diminished release of AA from PBMC. The inhibitory effect of GA on the AA release is a new element in the antiinflammatory pharmacological ability of this drug.

AB - The objective of this study was to investigate the effects of heat shock (HS) treatment and geldanamycin (GA) on the release of arachidonic acid (AA) from human peripheral blood mononuclear cells (PBMC), monocytes and lymphocytes. Mononuclear cells prepared from blood of healthy subjects were preincubated with 3H-AA. The release of 3H-AA incorporated into the membrane was studied after pretreatment of cells by HS (43°C, 1 h) and GA. The activation of AA producing enzymes was achieved by the addition of phorbol 12-myristate 13-acetate (PMA) or by the combination of PMA+calcium ionophore A-23187. Treatment of cells by HS inhibited the release of AA. Furthermore, the release of AA by PBMC was dose dependently inhibited by GA. The combination of treatments by HS and GA augmented the inhibition of AA release. The HS response involves a diminished release of AA from PBMC. The inhibitory effect of GA on the AA release is a new element in the antiinflammatory pharmacological ability of this drug.

KW - Arachidonic acid

KW - Geldanamycin

KW - Heat shock

KW - Phospholipase A

UR - http://www.scopus.com/inward/record.url?scp=0036771692&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036771692&partnerID=8YFLogxK

U2 - 10.1016/S0165-2478(02)00103-7

DO - 10.1016/S0165-2478(02)00103-7

M3 - Article

VL - 83

SP - 181

EP - 185

JO - Immunology Letters

JF - Immunology Letters

SN - 0165-2478

IS - 3

ER -