Inhibition of 12(S)-hydroxyeicosatetraenoic acid [12(S)-HETE]binding to epidermal cells by ultraviolet-B

Lajos Kemény, Bernard Przybilla, Eva Gross, Petr Arenberger, Thomas Ruzicka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

12-hydroxyeicosatetraenoic acid (12-HETE), the main eicosanoid in skin, is assumed to have both pathophysiologic effects in inflammatory skin diseases such as psoriasis and atopic eczema and a physiologic role in the biology of cutaneous reparative processes. Because 12-HETE exerts its effects via specific high-affinity epidermal receptors, and ultraviolet-B (UV-B) is capable of modulating various cell-surface molecules, the effects of single and repeated UV-B irradiations on the 12(S)-HETE binding sites in a human epidermal cell line, SCL-II, were studied. UV-B (100-300 J/m2) induced a large decrease in 12(S)-HETE binding in a dose-dependent manner. The inhibition occurred after a latency period of 6 h, reached its maximum at 18 h and slowly declined thereafter. A single UV-B dose of 300 J/m2 or repeated irradiation with 50 J/m2 of UV-B resulted in a 70% decrease in the number of binding sites (Bmax), whereas receptor affinity remained unaffected. The modulation of epidermal 12-HETE receptors by UV-B may partly explain the therapeutic effects of UV-B, but possibly also contribute to photodamage to skin.

Original languageEnglish
Pages (from-to)1028-1031
Number of pages4
JournalJournal of Investigative Dermatology
Volume97
Issue number6
DOIs
Publication statusPublished - Dec 1991

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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