Infrared studies of water induced conformational changes in bacteriorhodopsin

G. Váró, L. Eisenstein

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Fourier transform infrared difference spectroscopy has been used to study the effect of water on the conformation of bacteriorhodopsin. The infrared spectra as a function of water content show a conformational change at about 0.06 g H2O/g bacteriorhodopsin. By an interference method the thickness of the sample was measured and shows similar behavior as a function of water content. This study gives insight into the process of water absorption by purple membrane. The observations are in good agreement with those found for other proteins.

Original languageEnglish
Pages (from-to)163-168
Number of pages6
JournalEuropean Biophysics Journal
Volume14
Issue number3
DOIs
Publication statusPublished - Jan 1987

Fingerprint

Bacteriorhodopsins
Water
Purple Membrane
Fourier Transform Infrared Spectroscopy
Proteins

Keywords

  • bacteriorhodopsin
  • conformational changes
  • hydration
  • Infrared

ASJC Scopus subject areas

  • Biophysics

Cite this

Infrared studies of water induced conformational changes in bacteriorhodopsin. / Váró, G.; Eisenstein, L.

In: European Biophysics Journal, Vol. 14, No. 3, 01.1987, p. 163-168.

Research output: Contribution to journalArticle

@article{ae695f2809f444349076c495a0002a8c,
title = "Infrared studies of water induced conformational changes in bacteriorhodopsin",
abstract = "Fourier transform infrared difference spectroscopy has been used to study the effect of water on the conformation of bacteriorhodopsin. The infrared spectra as a function of water content show a conformational change at about 0.06 g H2O/g bacteriorhodopsin. By an interference method the thickness of the sample was measured and shows similar behavior as a function of water content. This study gives insight into the process of water absorption by purple membrane. The observations are in good agreement with those found for other proteins.",
keywords = "bacteriorhodopsin, conformational changes, hydration, Infrared",
author = "G. V{\'a}r{\'o} and L. Eisenstein",
year = "1987",
month = "1",
doi = "10.1007/BF00253841",
language = "English",
volume = "14",
pages = "163--168",
journal = "European Biophysics Journal",
issn = "0175-7571",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Infrared studies of water induced conformational changes in bacteriorhodopsin

AU - Váró, G.

AU - Eisenstein, L.

PY - 1987/1

Y1 - 1987/1

N2 - Fourier transform infrared difference spectroscopy has been used to study the effect of water on the conformation of bacteriorhodopsin. The infrared spectra as a function of water content show a conformational change at about 0.06 g H2O/g bacteriorhodopsin. By an interference method the thickness of the sample was measured and shows similar behavior as a function of water content. This study gives insight into the process of water absorption by purple membrane. The observations are in good agreement with those found for other proteins.

AB - Fourier transform infrared difference spectroscopy has been used to study the effect of water on the conformation of bacteriorhodopsin. The infrared spectra as a function of water content show a conformational change at about 0.06 g H2O/g bacteriorhodopsin. By an interference method the thickness of the sample was measured and shows similar behavior as a function of water content. This study gives insight into the process of water absorption by purple membrane. The observations are in good agreement with those found for other proteins.

KW - bacteriorhodopsin

KW - conformational changes

KW - hydration

KW - Infrared

UR - http://www.scopus.com/inward/record.url?scp=0023086126&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023086126&partnerID=8YFLogxK

U2 - 10.1007/BF00253841

DO - 10.1007/BF00253841

M3 - Article

VL - 14

SP - 163

EP - 168

JO - European Biophysics Journal

JF - European Biophysics Journal

SN - 0175-7571

IS - 3

ER -