The adherence of enterohemorrhagic Escherichia coli serotype 0157:H7 and various genetic derivatives to Henle 407 intestinal and HEp-2 epithelial cell lines was examined by light and electron microscopy. The parent outbreak strain, 7785, harbors a 60-megadalton serotype-specific plasmid designated pO157 and adhered to both cell lines, as determined by light microscopy. A plasmidelss derivative, 2-45, showed reduced adherence to both cell lines. After being labeled with Tn801, pO157 was transformed into E. coli C600, E. coli HB101, and E. coli GH42, and back into 2-45. Both E. coli C600 and HB101 transformants adhered weakly; full adherence was restored to the 2-45(pO157::Tn801) transformant. Transmission electron microscopy (EM) demonstrated the intimate attachment of HB101(pO157::Tn801) to Henle 407 cells which formed cuplike structures and areas of possible actin polymerization adjacent to adhering bacterial cells; scanning EM further extended these observations. EM studies of E. coli O157:H7 strains were hampered by extensive intestinal cell damage, presumably due to the action of Shiga-like toxins. EM also demonstrated that 7785 and its plasmidless derivatives 2-45 were pilated and that no pili were apparent on HB101(pO157::Tn801) or GH42//(pO157::Tn801). The plasmid pO157 appears to modify the eucaryotic cell adherence of E. coli O157:H7 and to confer that adherence of E. coli HB101 through surface structures other than pili. These findings, when compared with other published reports, also similarities between enterhmorrhagic and enteropathogenic E. coli adherence properties.
|Number of pages||9|
|Journal||Infection and Immunity|
|Publication status||Published - 1990|
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