Influence of Temperature on the Sieving Effect of Different Polymer Matrices in Capillary SDS Gel Electrophoresis of Proteins

András Guttman, Judit Horváth, Nelson Cooke

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Two different sieving polymers, branched (dextran) and linear (poly(ethylene oxide)), were used In high-performance capillary gel electrophoresis to study the effect of temperature on the separation of SDS-protein complexes In the molecular mass range of 14 400-97 400 Da. Migration properties and band broadening of the proteins were studied as a function of column temperature ranging from 20 and 50 °C. Equations have been derived that relate the effect of temperature on the mobility of the solute. Using an Isoelectrostatlc separation mode, the migration time of the SDS-protein molecules decreased In both Instances as temperature Increased within the examined time Interval. Peak efficiency Increased with elevated temperature In the dextran-based column but decreased when PEO was used as a sieving material.

Original languageEnglish
Pages (from-to)199-203
Number of pages5
JournalAnalytical Chemistry
Volume65
Issue number3
DOIs
Publication statusPublished - Feb 1 1993

ASJC Scopus subject areas

  • Analytical Chemistry

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