Influence of sampling and storage conditions on plasma renin activity and plasma renin concentration

Zoltan Locsei, K. Rácz, A. Patócs, G. Kovács, Erzsebet Toldy

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Because no systematic study on the comparison of the stability of plasma renin activity (PRA) and renin concentration (REN) under different preanalytical conditions has been reported, we examined the impact of several sampling and storage conditions. Methods: Blood samples were collected into prechilled tubes and into tubes at room temperature and processed within 30 min or kept at room temperature or at 0-5 °C for 2 h. The stability of PRA and REN after storage at - 20 °C was also analysed. PRA and REN were determined with radioimmunoassay (DiaSorin) and chemiluminescent immunometric method (LIAISON, DiaSorin), respectively. Results: When blood samples were kept at room temperature for 2 h, the PRA and REN decreased significantly (p <0.001), but both remained unchanged if samples were placed at 0-5 °C for the same time period. The REN was significantly higher in samples collected in prechilled tubes and refrigerated within 30 min at 0-5 °C compared to samples processed at room temperature (p <0.05). During storage at - 20 °C for 7 weeks the PRA decreased by 31.9 ± 4.7%, while the change in REN remained below 5%. Conclusion: Different sampling and storage conditions differentially influence the PRA and REN, which makes it necessary to apply well-defined preanalytical procedures for PRA and REN measurements.

Original languageEnglish
Pages (from-to)203-205
Number of pages3
JournalClinica Chimica Acta
Volume402
Issue number1-2
DOIs
Publication statusPublished - Apr 2009

Fingerprint

Renin
Thermodynamic properties
Sampling
Plasmas
Temperature
Blood
Radioimmunoassay

Keywords

  • Plasma renin activity
  • Renin concentration
  • Sampling
  • Storage

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Influence of sampling and storage conditions on plasma renin activity and plasma renin concentration. / Locsei, Zoltan; Rácz, K.; Patócs, A.; Kovács, G.; Toldy, Erzsebet.

In: Clinica Chimica Acta, Vol. 402, No. 1-2, 04.2009, p. 203-205.

Research output: Contribution to journalArticle

@article{59c8664bdc4b4ebdae8b93a3e01f338f,
title = "Influence of sampling and storage conditions on plasma renin activity and plasma renin concentration",
abstract = "Background: Because no systematic study on the comparison of the stability of plasma renin activity (PRA) and renin concentration (REN) under different preanalytical conditions has been reported, we examined the impact of several sampling and storage conditions. Methods: Blood samples were collected into prechilled tubes and into tubes at room temperature and processed within 30 min or kept at room temperature or at 0-5 °C for 2 h. The stability of PRA and REN after storage at - 20 °C was also analysed. PRA and REN were determined with radioimmunoassay (DiaSorin) and chemiluminescent immunometric method (LIAISON, DiaSorin), respectively. Results: When blood samples were kept at room temperature for 2 h, the PRA and REN decreased significantly (p <0.001), but both remained unchanged if samples were placed at 0-5 °C for the same time period. The REN was significantly higher in samples collected in prechilled tubes and refrigerated within 30 min at 0-5 °C compared to samples processed at room temperature (p <0.05). During storage at - 20 °C for 7 weeks the PRA decreased by 31.9 ± 4.7{\%}, while the change in REN remained below 5{\%}. Conclusion: Different sampling and storage conditions differentially influence the PRA and REN, which makes it necessary to apply well-defined preanalytical procedures for PRA and REN measurements.",
keywords = "Plasma renin activity, Renin concentration, Sampling, Storage",
author = "Zoltan Locsei and K. R{\'a}cz and A. Pat{\'o}cs and G. Kov{\'a}cs and Erzsebet Toldy",
year = "2009",
month = "4",
doi = "10.1016/j.cca.2009.01.013",
language = "English",
volume = "402",
pages = "203--205",
journal = "Clinica Chimica Acta",
issn = "0009-8981",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Influence of sampling and storage conditions on plasma renin activity and plasma renin concentration

AU - Locsei, Zoltan

AU - Rácz, K.

AU - Patócs, A.

AU - Kovács, G.

AU - Toldy, Erzsebet

PY - 2009/4

Y1 - 2009/4

N2 - Background: Because no systematic study on the comparison of the stability of plasma renin activity (PRA) and renin concentration (REN) under different preanalytical conditions has been reported, we examined the impact of several sampling and storage conditions. Methods: Blood samples were collected into prechilled tubes and into tubes at room temperature and processed within 30 min or kept at room temperature or at 0-5 °C for 2 h. The stability of PRA and REN after storage at - 20 °C was also analysed. PRA and REN were determined with radioimmunoassay (DiaSorin) and chemiluminescent immunometric method (LIAISON, DiaSorin), respectively. Results: When blood samples were kept at room temperature for 2 h, the PRA and REN decreased significantly (p <0.001), but both remained unchanged if samples were placed at 0-5 °C for the same time period. The REN was significantly higher in samples collected in prechilled tubes and refrigerated within 30 min at 0-5 °C compared to samples processed at room temperature (p <0.05). During storage at - 20 °C for 7 weeks the PRA decreased by 31.9 ± 4.7%, while the change in REN remained below 5%. Conclusion: Different sampling and storage conditions differentially influence the PRA and REN, which makes it necessary to apply well-defined preanalytical procedures for PRA and REN measurements.

AB - Background: Because no systematic study on the comparison of the stability of plasma renin activity (PRA) and renin concentration (REN) under different preanalytical conditions has been reported, we examined the impact of several sampling and storage conditions. Methods: Blood samples were collected into prechilled tubes and into tubes at room temperature and processed within 30 min or kept at room temperature or at 0-5 °C for 2 h. The stability of PRA and REN after storage at - 20 °C was also analysed. PRA and REN were determined with radioimmunoassay (DiaSorin) and chemiluminescent immunometric method (LIAISON, DiaSorin), respectively. Results: When blood samples were kept at room temperature for 2 h, the PRA and REN decreased significantly (p <0.001), but both remained unchanged if samples were placed at 0-5 °C for the same time period. The REN was significantly higher in samples collected in prechilled tubes and refrigerated within 30 min at 0-5 °C compared to samples processed at room temperature (p <0.05). During storage at - 20 °C for 7 weeks the PRA decreased by 31.9 ± 4.7%, while the change in REN remained below 5%. Conclusion: Different sampling and storage conditions differentially influence the PRA and REN, which makes it necessary to apply well-defined preanalytical procedures for PRA and REN measurements.

KW - Plasma renin activity

KW - Renin concentration

KW - Sampling

KW - Storage

UR - http://www.scopus.com/inward/record.url?scp=60649099046&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60649099046&partnerID=8YFLogxK

U2 - 10.1016/j.cca.2009.01.013

DO - 10.1016/j.cca.2009.01.013

M3 - Article

C2 - 19263530

AN - SCOPUS:60649099046

VL - 402

SP - 203

EP - 205

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

IS - 1-2

ER -