Influence of salt and pH on the hydrophobicity parameters of antisense nucleosides studied by reversed-phase thin-layer chromatography and multivariate mathematical-statistical methods

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The lipophilicity and specific hydrophobic surface area of 12 8-substituted 2'-deoxyadenosine and 17 5-substituted-2'-deoxyuridine derivatives were determined by reversed-phase thin-layer chromatography in ion-free eluents and in eluents containing sodium chloride, sodium acetate and acetic acid. The strength and selectivity of the effect of eluent additives were separated by use of spectral mapping technique followed by two-dimensional nonlinear mapping. The relationship between the structural characteristics and hydrophobicity parameters was elucidated by stepwise regression analysis. Eluent additives exert a considerable influence on both hydrophobicity parameters. The effect of sodium chloride and acetic acid was higher than that of sodium acetate. The strength and selectivity of the sensitivity of nucleosides towards eluent additives significantly depended on the character of the ring structure and on the length of the apolar alkyl chain. The influence of the degree of unsaturation and the branching of the alkyl substituent was negligible. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)21-30
Number of pages10
JournalJournal of Biochemical and Biophysical Methods
Volume41
Issue number1
DOIs
Publication statusPublished - Aug 30 1999

Fingerprint

Sodium Acetate
Thin layer chromatography
Reverse-Phase Chromatography
Hydrophobicity
Thin Layer Chromatography
Hydrophobic and Hydrophilic Interactions
Nucleosides
Sodium Chloride
Acetic Acid
Statistical methods
Salts
Deoxyuridine
Regression Analysis
Ions
Regression analysis
Derivatives
2'-deoxyadenosine

Keywords

  • Antisense nucleosides
  • Lipophilicity
  • Spectral mapping

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

@article{820a0e3fb6b948ab993e589806543d12,
title = "Influence of salt and pH on the hydrophobicity parameters of antisense nucleosides studied by reversed-phase thin-layer chromatography and multivariate mathematical-statistical methods",
abstract = "The lipophilicity and specific hydrophobic surface area of 12 8-substituted 2'-deoxyadenosine and 17 5-substituted-2'-deoxyuridine derivatives were determined by reversed-phase thin-layer chromatography in ion-free eluents and in eluents containing sodium chloride, sodium acetate and acetic acid. The strength and selectivity of the effect of eluent additives were separated by use of spectral mapping technique followed by two-dimensional nonlinear mapping. The relationship between the structural characteristics and hydrophobicity parameters was elucidated by stepwise regression analysis. Eluent additives exert a considerable influence on both hydrophobicity parameters. The effect of sodium chloride and acetic acid was higher than that of sodium acetate. The strength and selectivity of the sensitivity of nucleosides towards eluent additives significantly depended on the character of the ring structure and on the length of the apolar alkyl chain. The influence of the degree of unsaturation and the branching of the alkyl substituent was negligible. Copyright (C) 1999 Elsevier Science B.V.",
keywords = "Antisense nucleosides, Lipophilicity, Spectral mapping",
author = "T. Cserh{\'a}ti and E. Forg{\'a}cs",
year = "1999",
month = "8",
day = "30",
doi = "10.1016/S0165-022X(99)00029-9",
language = "English",
volume = "41",
pages = "21--30",
journal = "Journal of Proteomics",
issn = "1874-3919",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Influence of salt and pH on the hydrophobicity parameters of antisense nucleosides studied by reversed-phase thin-layer chromatography and multivariate mathematical-statistical methods

AU - Cserháti, T.

AU - Forgács, E.

PY - 1999/8/30

Y1 - 1999/8/30

N2 - The lipophilicity and specific hydrophobic surface area of 12 8-substituted 2'-deoxyadenosine and 17 5-substituted-2'-deoxyuridine derivatives were determined by reversed-phase thin-layer chromatography in ion-free eluents and in eluents containing sodium chloride, sodium acetate and acetic acid. The strength and selectivity of the effect of eluent additives were separated by use of spectral mapping technique followed by two-dimensional nonlinear mapping. The relationship between the structural characteristics and hydrophobicity parameters was elucidated by stepwise regression analysis. Eluent additives exert a considerable influence on both hydrophobicity parameters. The effect of sodium chloride and acetic acid was higher than that of sodium acetate. The strength and selectivity of the sensitivity of nucleosides towards eluent additives significantly depended on the character of the ring structure and on the length of the apolar alkyl chain. The influence of the degree of unsaturation and the branching of the alkyl substituent was negligible. Copyright (C) 1999 Elsevier Science B.V.

AB - The lipophilicity and specific hydrophobic surface area of 12 8-substituted 2'-deoxyadenosine and 17 5-substituted-2'-deoxyuridine derivatives were determined by reversed-phase thin-layer chromatography in ion-free eluents and in eluents containing sodium chloride, sodium acetate and acetic acid. The strength and selectivity of the effect of eluent additives were separated by use of spectral mapping technique followed by two-dimensional nonlinear mapping. The relationship between the structural characteristics and hydrophobicity parameters was elucidated by stepwise regression analysis. Eluent additives exert a considerable influence on both hydrophobicity parameters. The effect of sodium chloride and acetic acid was higher than that of sodium acetate. The strength and selectivity of the sensitivity of nucleosides towards eluent additives significantly depended on the character of the ring structure and on the length of the apolar alkyl chain. The influence of the degree of unsaturation and the branching of the alkyl substituent was negligible. Copyright (C) 1999 Elsevier Science B.V.

KW - Antisense nucleosides

KW - Lipophilicity

KW - Spectral mapping

UR - http://www.scopus.com/inward/record.url?scp=0345426354&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345426354&partnerID=8YFLogxK

U2 - 10.1016/S0165-022X(99)00029-9

DO - 10.1016/S0165-022X(99)00029-9

M3 - Article

C2 - 10512036

AN - SCOPUS:0345426354

VL - 41

SP - 21

EP - 30

JO - Journal of Proteomics

JF - Journal of Proteomics

SN - 1874-3919

IS - 1

ER -