Influence of precursors and additives on microbial lipases stabilized by sol-gel entrapment

Francisc Péter, László Poppe, Claudia Kiss, Emese Szocs-Bíró, Gabriela Preda, Cristina Zarcula, Alina Olteanu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Sol-gel entrapment of microbial lipases from Candida cylindracea (Cc lipase), Pseudomonas fluorescens (Lipase AK), and Pseudomonas cepacia (Lipase PS), using as precursors tetraethoxysilane (TEOS) and silanes of type R-Si(OEt)3 with alkyl or aryl R groups, has been investigated. Three different methods using these precursors were tried exhibiting protein immobilization yields in the range of 20-50%. Hydrolysis of emulsified olive oil, esterification of lauric acid with 1-octanol and enantioselective acylation of 2-pentanol have been used as model reactions for testing the properties of the encapsulated lipases. The recovery yields of the enzyme activity in the esterification reaction were between 20-68%, the best performance being achieved with phenyltriethoxysilane and tetraethoxysilane precursors at 3:1 molar ratio. When testing the entrapped Lipase AK in the enantioselective acylation reaction of 2-pentanol, activity recovery yields up to 32% related to the free enzyme were obtained and the immobilization increased the enantioselectivity of the enzyme.

Original languageEnglish
Pages (from-to)251-260
Number of pages10
JournalBiocatalysis and Biotransformation
Volume23
Issue number3-4
DOIs
Publication statusPublished - May 1 2005

Keywords

  • Enantioselectivity
  • Microbial lipase
  • Silane precursor
  • Sol-gel entrapment

ASJC Scopus subject areas

  • Biotechnology
  • Catalysis
  • Biochemistry

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