Influence of pinealectomy on levels of PACAP and cAMP in the chicken brain

Anikó Somogyvári-Vigh, R. Józsa, D. Reglodi, Tibor Hollósy, Róbert Meggyesi, I. Lengvári, Akira Arimura

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

One of the recently found functions of pituitary adenylate cyclase activating polypeptide (PACAP) is the modulation of circadian rhythms. Widespread distribution of PACAP-containing neurons and receptors has been shown in the chicken. Recently, we have demonstrated that PACAP levels oscillate in a circadian manner in the chicken brain. Daily variation in PACAP levels might be influenced by several regulatory mechanisms. Among the structures that may regulate PACAP levels, one candidate is the pineal gland. Therefore, in the present study, we investigated the effect of pinealectomy on the levels of PACAP in the chicken brain. Animals were kept under 12:12-h light-dark schedule. Pinealectomy was performed at 3 weeks of age; sham-operated animals were used as controls. The animals were sacrificed at 15 and 24 h 1 week after pinealectomy. The brainstem and diencephalon were removed, and tissue samples were processed for PACAP and cAMP radioimmunoassay (RIA). PACAP and cAMP levels showed nighttime elevations in both the sham-operated and pinealectomized animals, except for the PACAP content in the diencephalon of pinealectomized chicken. PACAP levels of pinealectomized animals were significantly higher in the diencephalon and brainstem as compared to the control animals at both time-points. Levels of cAMP correlated well with levels of PACAP. The present results provide evidence that the pineal gland has an inhibitory impact on PACAP-neurons in the chicken brainstem and diencephalon.

Original languageEnglish
Pages (from-to)9-13
Number of pages5
JournalRegulatory Peptides
Volume109
Issue number1-3
DOIs
Publication statusPublished - Nov 15 2002

Fingerprint

Pituitary Adenylate Cyclase-Activating Polypeptide
Chickens
Brain
Diencephalon
Animals
Brain Stem
Pineal Gland
Neurons
Circadian Rhythm
Radioimmunoassay
Appointments and Schedules

Keywords

  • cAMP
  • Chicken
  • PACAP
  • Pinealectomy

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Influence of pinealectomy on levels of PACAP and cAMP in the chicken brain. / Somogyvári-Vigh, Anikó; Józsa, R.; Reglodi, D.; Hollósy, Tibor; Meggyesi, Róbert; Lengvári, I.; Arimura, Akira.

In: Regulatory Peptides, Vol. 109, No. 1-3, 15.11.2002, p. 9-13.

Research output: Contribution to journalArticle

Somogyvári-Vigh, Anikó ; Józsa, R. ; Reglodi, D. ; Hollósy, Tibor ; Meggyesi, Róbert ; Lengvári, I. ; Arimura, Akira. / Influence of pinealectomy on levels of PACAP and cAMP in the chicken brain. In: Regulatory Peptides. 2002 ; Vol. 109, No. 1-3. pp. 9-13.
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