Influence of oxidative injury and monitoring of blood plasma by DSC on breast cancer patients

István Zapf, Medhi Moezzi, Tamás Fekecs, Klára Nedvig, D. Lőrinczy, A. Ferencz

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Damage caused by oxidative stress is involved in many types of diseases, including breast cancer. Our aim was to detect the oxidative stress parameters and blood plasma changes with differential scanning calorimetry (DSC) in breast cancer patients. The study included 40 adult breast cancer women who were grouped according to tumor diameter, regional lymph node metastases, proliferative activity, receptor status and postoperative chemotherapy. To monitor oxidative stress, malondialdehyde, oxygen free radicals (OFRs), activity of myeloperoxidase (MPO), superoxide dismutase (SOD) and catalase (CAT) were measured. Denaturation of plasma components was detected in Setaram Micro DSC-II calorimeter. The total production of OFRs, the MPO activity and lipidperoxidation were significantly increased in each breast cancer patients considering the tumor size, the metastatic lymph nodes, the proliferation activity and receptor status compared with healthy controls (p <0.05). These pro-oxidants were slightly elevated without chemotherapy, but their values were increased significantly in chemotherapy-receiving group. The activity of SOD and CAT was significantly decreased in all groups, and in regard to the chemotherapy, they were changed significantly parallel to the severity of disease. Regarding to both the increased tumor diameter and the increased number of affected lymph nodes, DSC measurements showed a strong relationship between the maximum excess heat capacity (Cpmax) of the blood plasma and the severity of disease. The study demonstrated that oxidative stress is implicated in breast carcinoma and chemotherapy aggravates these changes which confirmed the plasma DSC measurements also.

Original languageEnglish
JournalJournal of Thermal Analysis and Calorimetry
DOIs
Publication statusAccepted/In press - Mar 26 2015

Fingerprint

blood plasma
Chemotherapy
chemotherapy
Oxidative stress
breast
Differential scanning calorimetry
Blood
heat measurement
cancer
lymphatic system
Plasmas
scanning
Monitoring
Tumors
catalase
tumors
inorganic peroxides
Catalase
free radicals
Peroxidase

Keywords

  • Breast cancer
  • Diagnosis
  • DSC
  • Monitoring
  • Oxidative stress

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Condensed Matter Physics

Cite this

Influence of oxidative injury and monitoring of blood plasma by DSC on breast cancer patients. / Zapf, István; Moezzi, Medhi; Fekecs, Tamás; Nedvig, Klára; Lőrinczy, D.; Ferencz, A.

In: Journal of Thermal Analysis and Calorimetry, 26.03.2015.

Research output: Contribution to journalArticle

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