Influence of nicotine, cotinine, anabasine and cigarette smoke extract on human granulosa cell progesterone and estradiol synthesis

P. M. Gocze, I. Szabó, D. A. Freeman

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

To reveal the well known effect of smoking on the incidence of early abortion, the possible effects of cigarette alkaloids on progesterone and estradiol synthesis were investigated. A suspected cause for early spontaneous abortion is corpus luteum insufficiency. The present experiments evaluate the effects of cigarette smoke alkaloids on progesterone and estradiol biosyntheses. Human granulosa cells were obtained from patients undergoing in vitro fertilization and embryo transfer treatment because of infertility. Incubation of the granulosa cells with cotinine, anabasine, with the combination of nicotine, cotinine and anabasine, or with an aqueous extract of cigarette smoke resulted in inhibition of progesterone synthesis. The alkaloids and smoke extract decreased the DNA content of the culture dish. These latter findings suggested a cytotoxic effect of the alkaloids. Both cotinine and anabasine slightly stimulated the syntheses of normalized estradiol. However, nicotine, combination of all three alkaloids, and cigarette smoke extract had no significant influence on estradiol production. Taken together, these data would suggest that cigarette alkaloids inhibit cellular progesterone synthesis both by inhibiting progesterone synthesis and by causing less specific toxic effects to the cell. In contrast, cigarette smoke alkaloids slightly stimulated or had no effect on estradiol production. These concomitant actions of cigarette alkaloids partly explain the higher incidence of early abortion in pregnant women who smoke.

Original languageEnglish
Pages (from-to)266-272
Number of pages7
JournalGynecological Endocrinology
Volume13
Issue number4
Publication statusPublished - 1999

Fingerprint

Anabasine
Cotinine
Granulosa Cells
Nicotine
Alkaloids
Smoke
Tobacco Products
Progesterone
Estradiol
Poisons
Embryo Transfer
Corpus Luteum
Incidence
Spontaneous Abortion
Fertilization in Vitro
Infertility
Pregnant Women
Smoking

Keywords

  • Cigarette alkaloids
  • Cytotoxic effect
  • Estradiol
  • Human granulosa cells
  • Progesterone

ASJC Scopus subject areas

  • Endocrinology
  • Obstetrics and Gynaecology

Cite this

Influence of nicotine, cotinine, anabasine and cigarette smoke extract on human granulosa cell progesterone and estradiol synthesis. / Gocze, P. M.; Szabó, I.; Freeman, D. A.

In: Gynecological Endocrinology, Vol. 13, No. 4, 1999, p. 266-272.

Research output: Contribution to journalArticle

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