Influence of molecular configuration and conformation on the electromigration of oligosaccharides in narrow bore capillaries

Stefan Mittermayr, A. Guttman

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Capillary electrophoresis enables fast, high efficiency separations of oligosaccharides, wherein positional and/or linkage isomers, bearing the same charge-to-mass ratio, can readily be separated based on hydrodynamic radius differences. Fundamental electrophoretic mobility theory was used to investigate the correlation between changes in hydrodynamic volume equivalent radius and corresponding electrophoretic characteristics of oligosaccharides with different molecular properties. Fluorescently derivatized isomeric malto-, cello-, and isomaltooligosaccharide ladders, differing only in their linkage type of α1→4, β1→4, and α1→6, respectively, as well as a sterically larger N-acetylchitooligosaccharide ladder were used as model compounds. Mere differences in glycosidic linkage type or anomericity of isomeric oligo-glucoses had a decisive impact on their electromigration behavior, thus reflecting discrepancies in hydrodynamic radii and associated molecular conformations. The impact of hydrogen bridges, and associated availability of hydroxyl groups, on the molecular conformations, was investigated by hydrophilic interaction liquid chromatography. The experimentally observed electrophoretic and chromatographic differences between isomeric oligo-glucoses strongly suggested that special attention must be given when homooligosaccharide ladders are employed for normalization and comparability purposes, for example, in glucose unit calculation based structural elucidation.

Original languageEnglish
Pages (from-to)1000-1007
Number of pages8
JournalElectrophoresis
Volume33
Issue number6
DOIs
Publication statusPublished - Mar 2012

Fingerprint

Molecular Conformation
Electromigration
Ladders
Hydrodynamics
Oligosaccharides
Conformations
Glucose
Bearings (structural)
Capillary electrophoresis
Electrophoretic mobility
Liquid chromatography
Capillary Electrophoresis
Hydrophobic and Hydrophilic Interactions
Liquid Chromatography
Isomers
Hydroxyl Radical
Hydrogen
Availability

Keywords

  • Capillary electrophoresis
  • Hydrodynamic volume
  • Hydrophilic interaction liquid chromatography
  • Molecular conformation
  • Oligosaccharide analysis

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Influence of molecular configuration and conformation on the electromigration of oligosaccharides in narrow bore capillaries. / Mittermayr, Stefan; Guttman, A.

In: Electrophoresis, Vol. 33, No. 6, 03.2012, p. 1000-1007.

Research output: Contribution to journalArticle

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