Influence of in ovo administered Cryptosporidium baileyi oocyst extract on the course of homologous infection

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7 Citations (Scopus)

Abstract

In order to evaluate the effect of in ovo vaccination on avian cryptosporidiosis, two doses (1 and 10μg) of Cryptosporidium baileyi oocyst extract (OE) were injected into the amnionic sac of embryonated, specific pathogen-free chicken eggs. After hatching these birds as well as infected controls (IC) were inoculated with 8x105 C. baileyi oocysts at 10 days of age. Another group of chickens remained uninfected (UC). Faecal oocyst shedding was measured every second day, and weekly ELISAs were performed to monitor seroconversion. Those chickens that received OE during embryogenesis showed dose-dependent shift in their oocyst shedding, with higher oocyst output of OE1 and OE10 birds compared to IC ones. The patency was significantly longer in the OE10 group than in IC or OE1. ELISA results showed low seroconversion of OE1 and OE10 chickens prior to homologous challenge. Challenge infection resulted in antibody levels without significant difference between IC, OE1 and OE10 groups. These data suggest that in ovo vaccination with C. baileyi oocyst extract does not promote immune response, moreover, it may impair immunity and thus delay the clearance of cryptosporidia from chickens. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)313-319
Number of pages7
JournalVeterinary Parasitology
Volume89
Issue number4
DOIs
Publication statusPublished - May 17 2000

Fingerprint

Cryptosporidium baileyi
Cryptosporidium
Oocysts
oocysts
Chickens
extracts
Infection
infection
chickens
seroconversion
Birds
Vaccination
Enzyme-Linked Immunosorbent Assay
vaccination
enzyme-linked immunosorbent assay
Cryptosporidiosis
Specific Pathogen-Free Organisms
cryptosporidiosis
bird control
chicken eggs

Keywords

  • Chicken
  • Cryptosporidium baileyi
  • In ovo
  • Protozoa
  • Vaccine

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Parasitology
  • veterinary(all)

Cite this

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title = "Influence of in ovo administered Cryptosporidium baileyi oocyst extract on the course of homologous infection",
abstract = "In order to evaluate the effect of in ovo vaccination on avian cryptosporidiosis, two doses (1 and 10μg) of Cryptosporidium baileyi oocyst extract (OE) were injected into the amnionic sac of embryonated, specific pathogen-free chicken eggs. After hatching these birds as well as infected controls (IC) were inoculated with 8x105 C. baileyi oocysts at 10 days of age. Another group of chickens remained uninfected (UC). Faecal oocyst shedding was measured every second day, and weekly ELISAs were performed to monitor seroconversion. Those chickens that received OE during embryogenesis showed dose-dependent shift in their oocyst shedding, with higher oocyst output of OE1 and OE10 birds compared to IC ones. The patency was significantly longer in the OE10 group than in IC or OE1. ELISA results showed low seroconversion of OE1 and OE10 chickens prior to homologous challenge. Challenge infection resulted in antibody levels without significant difference between IC, OE1 and OE10 groups. These data suggest that in ovo vaccination with C. baileyi oocyst extract does not promote immune response, moreover, it may impair immunity and thus delay the clearance of cryptosporidia from chickens. Copyright (C) 2000 Elsevier Science B.V.",
keywords = "Chicken, Cryptosporidium baileyi, In ovo, Protozoa, Vaccine",
author = "S. Hornok and Z. Sz{\'e}ll and T. Sr{\'e}ter and A. Kov{\'a}cs and I. Varga",
year = "2000",
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T1 - Influence of in ovo administered Cryptosporidium baileyi oocyst extract on the course of homologous infection

AU - Hornok, S.

AU - Széll, Z.

AU - Sréter, T.

AU - Kovács, A.

AU - Varga, I.

PY - 2000/5/17

Y1 - 2000/5/17

N2 - In order to evaluate the effect of in ovo vaccination on avian cryptosporidiosis, two doses (1 and 10μg) of Cryptosporidium baileyi oocyst extract (OE) were injected into the amnionic sac of embryonated, specific pathogen-free chicken eggs. After hatching these birds as well as infected controls (IC) were inoculated with 8x105 C. baileyi oocysts at 10 days of age. Another group of chickens remained uninfected (UC). Faecal oocyst shedding was measured every second day, and weekly ELISAs were performed to monitor seroconversion. Those chickens that received OE during embryogenesis showed dose-dependent shift in their oocyst shedding, with higher oocyst output of OE1 and OE10 birds compared to IC ones. The patency was significantly longer in the OE10 group than in IC or OE1. ELISA results showed low seroconversion of OE1 and OE10 chickens prior to homologous challenge. Challenge infection resulted in antibody levels without significant difference between IC, OE1 and OE10 groups. These data suggest that in ovo vaccination with C. baileyi oocyst extract does not promote immune response, moreover, it may impair immunity and thus delay the clearance of cryptosporidia from chickens. Copyright (C) 2000 Elsevier Science B.V.

AB - In order to evaluate the effect of in ovo vaccination on avian cryptosporidiosis, two doses (1 and 10μg) of Cryptosporidium baileyi oocyst extract (OE) were injected into the amnionic sac of embryonated, specific pathogen-free chicken eggs. After hatching these birds as well as infected controls (IC) were inoculated with 8x105 C. baileyi oocysts at 10 days of age. Another group of chickens remained uninfected (UC). Faecal oocyst shedding was measured every second day, and weekly ELISAs were performed to monitor seroconversion. Those chickens that received OE during embryogenesis showed dose-dependent shift in their oocyst shedding, with higher oocyst output of OE1 and OE10 birds compared to IC ones. The patency was significantly longer in the OE10 group than in IC or OE1. ELISA results showed low seroconversion of OE1 and OE10 chickens prior to homologous challenge. Challenge infection resulted in antibody levels without significant difference between IC, OE1 and OE10 groups. These data suggest that in ovo vaccination with C. baileyi oocyst extract does not promote immune response, moreover, it may impair immunity and thus delay the clearance of cryptosporidia from chickens. Copyright (C) 2000 Elsevier Science B.V.

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KW - Cryptosporidium baileyi

KW - In ovo

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