Influence of imprinting with A and B chains of insulin on binding and functional changes in tetrahymena

G. Csaba, P. Kovács

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Insulin and its A and B chain increased the quantity of intracellular PAS-positive material (glycogen) in tetrahymena, whereas the combined A+B chains decreased it. Imprinting-previous interaction-with insulin, its A and B chains in themselves and with the A+B chain increased the hormone binding capacity of tetrahymena, but the functional effect of imprinting (storage or breakdown of glycogen) showed a different tendency with insulin and A+B chain on the one hand, and A chain and B chain on the other. Since the imprinting potential of a molecule promotes the induction of receptor formation, the fact remains that both component chains of insulin were able to act as potential imprinters, although the A chain was superior to the B chain in this respect throughout, and combined treatment with the A+B chain ultimately induced the formation of a similar binding site as insulin itself.

Original languageEnglish
Pages (from-to)431-436
Number of pages6
JournalBioscience Reports
Volume10
Issue number5
DOIs
Publication statusPublished - Oct 1 1990

Keywords

  • glucose storage
  • hormonal imprinting
  • insulin chains
  • receptors
  • tetrahymena

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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